Home LiteratureArticle Details
PMID: 1167153 Published · ppublish English Journal Article

Identification of amino acid thiohydantoins directly by thin-layer chromatography and indirectly by gas-liquid chromatography after hydrolysis.

The Biochemical journal ·Vol. 147 ·No. 3 ·1975-06-00 ·Pages 435-8

Rangarajan M, Darbre A

Abstract

A method is described for the identification of amino acid thiohydantoins by two-dimensional t.l.c. An indirect method for the determination of amino acid thiohydantoins is described which, after hydrolysis, the corresponding amino acids are determined by g.l.c.

MeSH Terms
Amino Acid Sequence Chromatography, Gas/methods Chromatography, Thin Layer/methods Hydantoins/analysis Hydrolysis Peptides/analysis Thiohydantoins/analysis
Chemicals
Hydantoins Peptides Thiohydantoins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rangarajan M
Darbre A
References (23)
23 references, click to expand
  1. The use of cyanate for the determination of NH2-terminal residues in proteins.
    J Biol Chem. 1963 Jan;238:214-26 PMID: 13983448
  2. Hydrolysis of phenylthiohydantoins of amino acids.
    Biochem Biophys Res Commun. 1964;14:399-403 PMID: 5836532
  3. Identification of C-terminal residues in peptides and proteins through formation of thiohydantoins.
    Biochim Biophys Acta. 1954 Apr;13(4):553-9 PMID: 13159994
  4. Rapid separation of phenylthiohydantoin (PTH) amino acids by thin-layer chromatography on polyamide glass plates.
    Anal Biochem. 1971 Dec;44(2):548-58 PMID: 5130945
  5. Determination of the amino acid sequence of the monkey, sheep, and dog proinsulin C-peptides by a semi-micro Edman degradation procedure.
    J Biol Chem. 1972 Aug 10;247(15):4866-71 PMID: 4626369
  6. Amino acid sequences of fifty residues from the amino termini of the tryptophan synthetase chains of several enterobacteria.
    J Biol Chem. 1972 Feb 25;247(4):1031-7 PMID: 4551507
  7. Thin-layer chromatography of methylthiohydantoin amino acids.
    J Chromatogr. 1974 Mar 13;90(1):226-9 PMID: 4824661
  8. Gas-liquid chromatography and mass spectrometry of amino acid thiohydantoins and their use in protein sequencing.
    J Chromatogr. 1973 Dec 19;87(2):499-512 PMID: 4765846
  9. Thin-layer chromatography of amino acid hydantoins.
    J Chromatogr. 1973 Jun;80(2):199-204 PMID: 4723044
  10. Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets.
    Anal Biochem. 1973 Jun;53(2):624-8 PMID: 4716391
  11. Advances in the gas chromatographic analysis of amino acid phenyl- and methylthiohydantoins.
    Anal Biochem. 1972 Jan;45(1):43-59 PMID: 5061852
  12. Gas-liquid chromatography of trifluoroacetylated amino acid methyl esters. Determination of their molar responses with the flame ionization detector.
    J Chromatogr. 1972 Sep 6;71(2):223-32 PMID: 5057113
  13. Determination of the carboxyl termini of proteins with ammonium thiocyanate and acetic anhydride, with direct identification of the thiohydantoins.
    Biochemistry. 1969 Dec;8(12):4735-40 PMID: 4904040
  14. Acid hydrolysis of phenylthiohydantoins of amino acids.
    Aust J Biol Sci. 1971 Dec;24(6):1247-50 PMID: 5163484
  15. Solid-phase Edman degradation. An automatic peptide sequencer.
    Eur J Biochem. 1971 May 11;20(1):89-102 PMID: 5578618
  16. Protein hydrolysis and chemical reactions using Rotaflo stopcocks.
    Lab Pract. 1971 Sep;20(9):726 PMID: 5092884
  17. Sequential degradation of polypeptides from the carboxyl ends. I. Specific cleavage of the carboxyl-end peptide bonds.
    Biochim Biophys Acta. 1971 Feb 16;229(2):301-9 PMID: 5553979
  18. Analysis of amino acid phenylthiohydantoins by gas chromatography.
    J Biol Chem. 1969 Oct 25;244(20):5597-607 PMID: 5348603
  19. Sequential degradation of peptides from their carboxyl termini with ammonium thiocyanate and acetic anhydride.
    Biochemistry. 1968 May;7(5):1796-807 PMID: 5689841
  20. Gas-liquid chromatography of trifluoroacetylated amino acid methyl esters.
    Biochem J. 1968 Feb;106(4):923-5 PMID: 5637373
  21. A protein sequenator.
    Eur J Biochem. 1967 Mar;1(1):80-91 PMID: 6059350
  22. A novel method for the determination of C-terminal amino acid in polypeptides by selective tritium labelling.
    Biochem Biophys Res Commun. 1966 Jan 4;22(1):69-74 PMID: 5937340
  23. Acid hydrolysis of 3-phenyl-2-thiohydantoins.
    Biochim Biophys Acta. 1954 Dec;15(4):589 PMID: 13230112
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1470435
Published
1975-06-00
Pages
435-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165469
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1470435 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2