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PMID: 11668343 Published · ppublish English Comparative Study Journal Article

Murine Schnurri-2 is required for positive selection of thymocytes.

Nature immunology ·Vol. 2 ·No. 11 ·2001-11-00 ·Pages 1048-53

Takagi T, Harada J, Ishii S

Abstract

A key step in T cell development involves the positive selection of cells that recognize antigen presented by self-major histocompatibility complex. Yet, the signals that are activated by T cell receptor engagement and lead to cell survival remain unclear. We show here that mice lacking the transcription factor Schnurri-2 (Shn-2), a large metal-finger protein, had severely defective positive selection of CD4+ and CD8+ cells. Drosophila Shn acts as a cofactor of Smad homolog and is required for Decapentaplegic signaling. Vertebrates have at least three Shn orthologs (Shn-1, Shn-2 and Shn-3), which are thought to act as nuclear targets in the bone morphogenetic protein-transforming growth factor-beta-activin signaling pathways. These data raised the possibility that the Smad-Shn-2 complex is involved in the thymic selection of T cells.

MeSH Terms
Activins/physiology Animals Bone Marrow Cells/metabolism Bone Marrow Transplantation Bone Morphogenetic Proteins/physiology CD4-Positive T-Lymphocytes/cytology,immunology CD8-Positive T-Lymphocytes/cytology,immunology Carrier Proteins/genetics,physiology Clonal Deletion/physiology DNA-Binding Proteins/deficiency,genetics,physiology Drosophila Proteins/physiology Drosophila melanogaster/embryology,genetics Epithelial Cells/cytology Female Gene Expression Regulation, Developmental Gene Targeting Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout NF-kappa B/metabolism Radiation Chimera Self Tolerance/physiology Signal Transduction Smad4 Protein Species Specificity Spleen/immunology,pathology Stromal Cells/metabolism T-Lymphocyte Subsets/immunology,pathology Thymus Gland/immunology,pathology,transplantation Trans-Activators/genetics,physiology Transcription Factors/physiology Transforming Growth Factor beta/physiology
Chemicals
Bone Morphogenetic Proteins Carrier Proteins DNA-Binding Proteins Drosophila Proteins HIVEP1 protein, human HIVEP3 protein, human Hivep3 protein, mouse MAD protein, Drosophila Med protein, Drosophila NF-kappa B Smad4 Protein Trans-Activators Transcription Factors Transforming Growth Factor beta dpp protein, Drosophila shn protein, Drosophila Activins HIVEP2 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takagi T
Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, CREST Research Project of JST, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
Harada J
Ishii S
Article Info
Journal
Nature immunology
Abbr.
Nat Immunol
ISSN
1529-2908
Published
2001-11-00
Pages
1048-53
Language
English
Region
United States
NLM ID
100941354
Subset
IM
Corrections
CommentIn
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