Tubulo-reticular clusters of peroxisomes in living COS-7 cells: dynamic behavior and association with lipid droplets.

We characterized more complex peroxisomal structures, i.e., tubulo-reticular peroxisomal clusters, in greater detail under in vivo conditions in COS-7 cells that were transfected with a GFP-PTS1 fusion protein. Live cell imaging revealed the dynamic nature of peroxisomal clusters and allowed a detailed analysis of the motile properties of a heterogeneous peroxisome population. Furthermore, peroxisomal clusters were found to be associated with lipid droplets. The frequency of peroxisomal clusters correlated with an increase in cell density and in the size of lipid droplets. These data provide further evidence for the dynamic nature of the peroxisomal compartment and indicate that peroxisomal clusters have a function in lipid metabolism.