An optomechanical transducer in the blue light receptor phototropin from Avena sativa.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 22 ·2001-10-23 ·Pages 12357-61

Salomon M, Eisenreich W, Dürr H, Schleicher E, Knieb E, Massey V, Rüdiger W, Müller F, Bacher A, Richter G

Abstract

The PHOT1 (NPH1) gene from Avena sativa specifies the blue light receptor for phototropism, phototropin, which comprises two FMN-binding LOV domains and a serine/threonine protein kinase domain. Light exposure is conducive to autophosphorylation of the protein kinase domain. We have reconstituted a recombinant LOV2 domain of A. sativa phototropin with various (13)C/(15)N-labeled isotopomers of the cofactor, FMN. The reconstituted protein samples were analyzed by NMR spectroscopy under dark and light conditions. Blue light irradiation is shown to result in the addition of a thiol group (cysteine 450) to the 4a position of the FMN chromophore. The adduct reverts spontaneously in the dark by elimination. The light-driven flavin adduct formation results in conformational modification, which was diagnosed by (1)H and (31)P NMR spectroscopy. This conformational change is proposed to initiate the transmission of the light signal via conformational modulation of the protein kinase domain conducive to autophosphorylation of NPH1.

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Salomon M

Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.

Eisenreich W

Dürr H

Schleicher E

Knieb E

Massey V

Rüdiger W

Müller F

Bacher A

Richter G

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.221455298

Published

2001-10-23

Epub

2001-00-16

Pages

12357-61

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC60058

External Links

PubMed source DOI: 10.1073/pnas.221455298 PMC Full Text View journal details

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An optomechanical transducer in the blue light receptor phototropin from Avena sativa.

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