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PMID: 11583621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of hTAF(II)80 delta links apoptotic signaling pathways to transcription factor TFIID function.

Molecular cell ·Vol. 8 ·No. 3 ·2001-09-00 ·Pages 591-600

Bell B, Scheer E, Tora L

Abstract

Apoptotic cell death is associated with altered levels of mRNA expression, yet the mechanisms that coordinate changes in gene expression with activation of the cell death machinery remain obscure. Here, we report the cloning and characterization of hTAF(II)80 delta, a specialized isoform of the general transcription factor TFIID subunit hTAF(II)80. Several distinct apoptotic stimuli induce the expression and caspase-dependent cleavage of hTAF(II)80 delta. hTAF(II)80 delta, unlike hTAF(II)80, forms a TFIID-like complex lacking hTAF(II)31. Elevated expression of hTAF(II)80 delta in HeLa cells is sufficient to trigger apoptotic cell death and selectively alters cellular transcription, including the induction of the target genes gadd45 and p21. These data define a signaling pathway that couples apoptotic signals to a reprogramming of RNA polymerase II transcription.

MeSH Terms
Amino Acid Sequence Apoptosis/drug effects,physiology Apoptosis Regulatory Proteins Camptothecin/pharmacology Caspase Inhibitors Caspases/metabolism Cell Line Cloning, Molecular Enzyme Inhibitors/pharmacology Etoposide/pharmacology Humans Immunoblotting Intracellular Signaling Peptides and Proteins Macromolecular Substances Membrane Glycoproteins/metabolism Molecular Sequence Data Nucleic Acid Synthesis Inhibitors/pharmacology Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerases Protein Isoforms/genetics,metabolism Proteins/genetics,metabolism Recombinant Fusion Proteins/metabolism Sequence Alignment Signal Transduction/physiology Staurosporine/pharmacology TATA-Binding Protein Associated Factors TNF-Related Apoptosis-Inducing Ligand Transcription Factor TFIID Transcription Factors/chemistry,genetics,metabolism Transcription Factors, TFII/genetics,metabolism Transcription, Genetic/physiology Tumor Necrosis Factor-alpha/metabolism rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Apoptosis Regulatory Proteins Caspase Inhibitors Enzyme Inhibitors GADD45 protein Intracellular Signaling Peptides and Proteins Macromolecular Substances Membrane Glycoproteins Nucleic Acid Synthesis Inhibitors Protein Isoforms Proteins Recombinant Fusion Proteins TAF6 protein, human TATA-Binding Protein Associated Factors TNF-Related Apoptosis-Inducing Ligand TNFSF10 protein, human Transcription Factor TFIID Transcription Factors Transcription Factors, TFII Tumor Necrosis Factor-alpha Etoposide PARP1 protein, human Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerases Caspases rho GTP-Binding Proteins Staurosporine Camptothecin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bell B
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163, F-67404 ILLKIRCH Cedex, C.U. de Strasbourg, France.
Scheer E
Tora L
Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-2765
Published
2001-09-00
Pages
591-600
Language
English
Region
United States
NLM ID
9802571
Subset
IM
Corrections
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