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PMID: 11581317 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Migratory properties of naive, effector, and memory CD8(+) T cells.

The Journal of experimental medicine ·Vol. 194 ·No. 7 ·2001-10-01 ·Pages 953-66

Weninger W, Crowley MA, Manjunath N, von Andrian UH

Abstract

It has been proposed that two different antigen-experienced T cell subsets may be distinguishable by their preferential ability to home to lymphoid organs (central memory cells) or nonlymphoid tissues (effector memory/effector cells). We have shown recently that murine antigen-primed CD8(+) T cells cultured in interleukin (IL)-15 (CD8(IL-15)) resemble central memory cells in phenotype and function. In contrast, primed CD8(+) T cells cultured in IL-2 (CD8(IL-2)) become cytotoxic effector cells. Here, the migratory behavior of these two subsets was investigated. Naive, CD8(IL-15) cells and, to a lesser degree, CD8(IL-2) cells localized to T cell areas in the spleen, but only naive and CD8(IL-15) cells homed to lymph nodes (LNs) and Peyer's patches. Intravital microscopy of peripheral LNs revealed that CD8(IL-15) cells, but not CD8(IL-2) cells, rolled and arrested in high endothelial venules (HEVs). Migration of CD8(IL-15) cells to LNs depended on L-selectin and required chemokines that bind CC chemokine receptor (CCR)7. Both antigen-experienced populations, but not naive T cells, responded to inflammatory chemokines and accumulated at sites of inflammation. However, CD8(IL-2) cells were 12 times more efficient in migrating to inflamed peritoneum than CD8(IL-15) cells. Furthermore, CD8(IL-15) cells proliferated rapidly upon reencounter with antigen at sites of inflammation. Thus, central memory-like CD8(IL-15) cells home avidly to lymphoid organs and moderately to sites of inflammation, where they mediate rapid recall responses, whereas CD8(IL-2) effector T cells accumulate in inflamed tissues, but are excluded from most lymphoid organs.

MeSH Terms
Animals Blood Circulation CD8-Positive T-Lymphocytes/immunology Cell Division Chemotaxis, Leukocyte/immunology Female Immunologic Memory Inflammation/immunology Interleukin-15/immunology Interleukin-2/immunology Lymph Nodes/cytology,immunology Lymphatic System/cytology,immunology Male Mice Peyer's Patches/cytology,immunology Spleen/cytology,immunology T-Lymphocyte Subsets/immunology
Chemicals
Interleukin-15 Interleukin-2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Weninger W
The Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA.
Crowley M A
Manjunath N
von Andrian U H
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.194.7.953
Published
2001-10-01
Pages
953-66
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2193483
Subset
IM
Grants
NHLBI NIH HHS · HL56949 · United States
NHLBI NIH HHS · HL48675 · United States
NHLBI NIH HHS · HL54936 · United States
External Links
PubMed source DOI: 10.1084/jem.194.7.953 PMC Full Text View journal details
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