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PMID: 11581287 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes.

The Journal of cell biology ·Vol. 155 ·No. 1 ·2001-10-01 ·Pages 77-88

Noda Y, Okada Y, Saito N, Setou M, Xu Y, Zhang Z, Hirokawa N

Abstract

We have identified and characterized a COOH-terminal motor domain-type kinesin superfamily protein (KIFC), KIFC3, in the kidney. KIFC3 is a minus end-directed microtubule motor protein, therefore it accumulates in regions where minus ends of microtubules assemble. In polarized epithelial cells, KIFC3 is localized on membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells in vivo and polarized MDCK II cells in vitro. Flotation assay, coupled with detergent extraction, demonstrated that KIFC3 is associated with Triton X-100-insoluble membrane organelles, and that it overlaps with apically transported TGN-derived vesicles. This was confirmed by immunoprecipitation and by GST pulldown experiments showing the specific colocalization of KIFC3 and annexin XIIIb, a previously characterized membrane protein for apically transported vesicles (Lafont, F., S. Lecat, P. Verkade, and K. Simons. 1998. J. Cell Biol. 142:1413-1427). Furthermore, we proved that the apical transport of both influenza hemagglutinin and annexin XIIIb was partially inhibited or accelerated by overexpression of motor-domainless (dominant negative) or full-length KIFC3, respectively. Absence of cytoplasmic dynein on these annexin XIIIb-associated vesicles and distinct distribution of the two motors on the EM level verified the existence of KIFC3-driven transport in epithelial cells.

MeSH Terms
Amino Acid Sequence Animals Annexins/metabolism Cell Line Cell Membrane/chemistry,metabolism Cell Polarity Cholesterol/metabolism Cloning, Molecular Consensus Sequence Detergents/chemistry Dogs Epithelial Cells/drug effects,metabolism Immunohistochemistry Kidney Tubules/metabolism,ultrastructure Kinesins/chemistry,genetics,metabolism Microtubules/metabolism Molecular Motor Proteins/chemistry,genetics,metabolism Molecular Sequence Data Nocodazole/pharmacology Octoxynol/chemistry Protein Isoforms/metabolism Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins/metabolism Sequence Alignment Temperature
Chemicals
Annexins Detergents Kifc3 protein, mouse Molecular Motor Proteins Protein Isoforms Recombinant Fusion Proteins Octoxynol Cholesterol Kinesins Nocodazole
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Noda Y
Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113-0033, Japan.
Okada Y
Saito N
Setou M
Xu Y
Zhang Z
Hirokawa N
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200108042
Published
2001-10-01
Pages
77-88
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2150803
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200108042 PMC Full Text View journal details
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