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PMID: 11576428 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Contrasting modes of evolution acting on the complex N locus for rust resistance in flax.

The Plant journal : for cell and molecular biology ·Vol. 27 ·No. 5 ·2001-09-00 ·Pages 439-53

Dodds PN, Lawrence GJ, Ellis JG

Abstract

Three rust resistance specificities, N, N1 and N2, map to the complex N locus of flax. We used a degenerate PCR approach, with primers directed to the nucleotide binding site (NBS) domain characteristic of many plant resistance genes, to isolate resistance gene analogs (RGAs) from flax. One RGA clone detected RFLPs co-segregating with alleles of the N locus. With this probe we isolated four related genes that occur within a 30kbp region and encode proteins with NBS and leucine-rich repeat (LRR) domains and N-terminal Toll/Interleukin-1 Receptor homology (TIR) domains. One of these four genes was identified as the N resistance gene by sequence analysis of three mutant alleles and by transgenic expression. We isolated homologous genes from two flax lines containing the N1 or N2 specificities and from flax lines carrying no N locus resistance specificities. Analysis of shared polymorphisms among this set of 18 N locus sequences revealed three groups of genes with independent lineages. Sequence exchanges have only occurred between genes within each group, but not between groups. Two of the groups contain only one sequence from each haplotype and probably represent orthologous genes. However, the third group contains two genes from each haplotype. We suggest that the re-assortment of variation by recombination/gene conversion at this locus is limited by the degree of sequence identity between genes.

MeSH Terms

Alleles Amino Acid Sequence Basidiomycota Biological Evolution Chromosome Segregation Drosophila Proteins Flax/genetics,microbiology Gene Conversion Genes, Plant Haplotypes Immunity, Innate/genetics Membrane Glycoproteins/genetics Models, Genetic Molecular Sequence Data Mutation Plant Diseases/genetics Plants, Genetically Modified Polymorphism, Genetic Polymorphism, Restriction Fragment Length Protein Structure, Tertiary RNA Splicing Receptors, Cell Surface/genetics Receptors, Interleukin-1/genetics Recombination, Genetic Selection, Genetic Sequence Homology, Amino Acid Toll-Like Receptors

Chemicals

Drosophila Proteins Membrane Glycoproteins Receptors, Cell Surface Receptors, Interleukin-1 Toll-Like Receptors

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dodds P N

CSIRO Plant Industry, Canberra ACT 2601, Australia.

Lawrence G J

Ellis J G

Article Info

Journal

The Plant journal : for cell and molecular biology

Abbr.

Plant J

ISSN

0960-7412

DOI

10.1046/j.1365-313x.2001.01114.x

Published

2001-09-00

Pages

439-53

Language

English

Region

England

NLM ID

9207397

Subset

Databases

GENBANK

AJ310150, AJ310151, AJ310152, AJ310153, AJ310154, AJ310155, AJ310156, AJ310157, AJ310158, AJ310159, AJ310160, AJ310161, AJ310162, AJ310163, AJ310164, AJ310165, AJ310168, AJ310169, AJ310170, AJ310171, AJ310172, AJ310173, AJ310174, AJ310175, AJ310176, AJ310177, AJ310178, AJ310179, AJ310180, AJ310181, AJ310182, AJ310183

External Links

PubMed source DOI: 10.1046/j.1365-313x.2001.01114.x View journal details

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Contrasting modes of evolution acting on the complex N locus for rust resistance in flax.

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