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PMID: 11553558 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

TonB-dependent systems of uropathogenic Escherichia coli: aerobactin and heme transport and TonB are required for virulence in the mouse.

Infection and immunity ·Vol. 69 ·No. 10 ·2001-10-00 ·Pages 6179-85

Torres AG, Redford P, Welch RA, Payne SM

Abstract

The uropathogenic Escherichia coli strain CFT073 has multiple iron acquisition systems, including heme and siderophore transporters. A tonB mutant derivative of CFT073 failed to use heme as an iron source or to utilize the siderophores enterobactin and aerobactin, indicating that transport of these compounds in CFT073 is TonB dependent. The TonB(-) derivative showed reduced virulence in a mouse model of urinary tract infection. Virulence was restored when the tonB gene was introduced on a plasmid. To determine the importance of the individual TonB-dependent iron transport systems during urinary tract infections, mutants defective in each of the CFT073 high-affinity iron transport systems were constructed and tested in the mouse model. Mouse virulence assays indicated that mutants defective in a single iron transport system were able to infect the kidney when inoculated as a pure culture but were unable to efficiently compete with the wild-type strain in mixed infections. These results indicate a role for TonB-dependent systems in the virulence of uropathogenic E. coli strains.

MeSH Terms
Animals Bacterial Proteins/genetics,metabolism,physiology Biological Transport Disease Models, Animal Escherichia coli/growth & development,metabolism,pathogenicity Escherichia coli Infections/microbiology Escherichia coli Proteins Heme/metabolism Hydroxamic Acids/metabolism Iron/metabolism Membrane Proteins/genetics,metabolism,physiology Mice Mutagenesis Siderophores/metabolism Urinary Tract Infections/microbiology Virulence
Chemicals
Bacterial Proteins Escherichia coli Proteins Hydroxamic Acids Membrane Proteins Siderophores tonB protein, Bacteria tonB protein, E coli aerobactin Heme Iron
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Torres A G
Department of Microbiology and the Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
Redford P
Welch R A
Payne S M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.69.10.6179-6185.2001
Published
2001-10-00
Pages
6179-85
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC98749
Subset
IM
Grants
NIAID NIH HHS · R01 AI016935 · United States
NIAID NIH HHS · AI01583 · United States
NIAID NIH HHS · AI16935 · United States
NIDDK NIH HHS · R01 DK063250 · United States
NIAID NIH HHS · AI39000 · United States
NIAID NIH HHS · R37 AI016935 · United States
External Links
PubMed source DOI: 10.1128/IAI.69.10.6179-6185.2001 PMC Full Text View journal details
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