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PMID: 11546875 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses.

Science (New York, N.Y.) ·Vol. 293 ·No. 5536 ·2001-09-07 ·Pages 1840-2

Hatta M, Gao P, Halfmann P, Kawaoka Y

Abstract

In 1997, an H5N1 influenza A virus was transmitted from birds to humans in Hong Kong, killing 6 of the 18 people infected. When mice were infected with the human isolates, two virulence groups became apparent. Using reverse genetics, we showed that a mutation at position 627 in the PB2 protein influenced the outcome of infection in mice. Moreover, high cleavability of the hemagglutinin glycoprotein was an essential requirement for lethal infection.

MeSH Terms

Amino Acid Sequence Animals Birds/virology DNA, Recombinant/genetics Hemagglutinin Glycoproteins, Influenza Virus/chemistry,genetics,metabolism Hong Kong/epidemiology Humans Influenza A Virus, H5N1 Subtype Influenza A virus/genetics,pathogenicity,physiology Influenza, Human/epidemiology,mortality,transmission,virology Lung/virology Mice Mutation, Missense/genetics RNA-Dependent RNA Polymerase Reassortant Viruses/genetics,pathogenicity,physiology Viral Proteins/chemistry,genetics,metabolism

Chemicals

DNA, Recombinant Hemagglutinin Glycoproteins, Influenza Virus PB2 protein, Influenzavirus A Viral Proteins RNA-Dependent RNA Polymerase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hatta M

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.

Gao P

Halfmann P

Kawaoka Y

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.1062882

Published

2001-09-07

Pages

1840-2

Language

English

Region

United States

NLM ID

0404511

Subset

Corrections

CommentIn

PMID: 11546856

External Links

PubMed source DOI: 10.1126/science.1062882 View journal details

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Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses.

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