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PMID: 11535825 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of melastatin, a TRP-related protein, through interaction with a cytoplasmic isoform.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 19 ·2001-09-11 ·Pages 10692-7

Xu XZ, Moebius F, Gill DL, Montell C

Abstract

The TRP (transient receptor potential) superfamily includes a group of subfamilies of channel-like proteins mediating a multitude of physiological signaling processes. The TRP-melastatin (TRPM) subfamily includes the putative tumor suppressor melastatin (MLSN) and is a poorly characterized group of TRP-related proteins. Here, we describe the identification and characterization of an additional TRPM protein TRPM4. We reveal that TRPM4 and MLSN each mediate Ca(2+) entry when expressed in HEK293 cells. Furthermore, we demonstrate that a short form of MLSN (MLSN-S) interacts directly with and suppresses the activity of full-length MLSN (MLSN-L). This suppression seems to result from the inhibition of translocation of MLSN-L to the plasma membrane. We propose that control of translocation through interaction between MLSN-S and MLSN-L represents a mode for regulating ion channel activity.

MeSH Terms
Amino Acid Sequence Calcium/metabolism Calcium Channels/genetics,metabolism Cation Transport Proteins Cell Line Cytoplasm/metabolism Humans Membrane Proteins/genetics,metabolism Molecular Sequence Data Neoplasm Proteins Protein Isoforms/metabolism TRPM Cation Channels
Chemicals
Calcium Channels Cation Transport Proteins Membrane Proteins Neoplasm Proteins Protein Isoforms TRPM Cation Channels TRPM1 protein, human TRPM4 protein, human Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Xu X Z
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Moebius F
Gill D L
Montell C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.191360198
Published
2001-09-11
Epub
2001-00-04
Pages
10692-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC58528
Subset
IM
Grants
NEI NIH HHS · R01 EY010852 · United States
NEI NIH HHS · EY10852 · United States
Databases
GENBANK
AY046396
External Links
PubMed source DOI: 10.1073/pnas.191360198 PMC Full Text View journal details
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