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PMID: 11533201 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The pathogenicity of human immunodeficiency virus (HIV) type 1 Nef in CD4C/HIV transgenic mice is abolished by mutation of its SH3-binding domain, and disease development is delayed in the absence of Hck.

Journal of virology ·Vol. 75 ·No. 19 ·2001-10-00 ·Pages 9378-92

Hanna Z, Weng X, Kay DG, Poudrier J, Lowell C, Jolicoeur P

Abstract

The human immunodeficiency virus type 1 (HIV-1) Nef protein is an important determinant of AIDS pathogenesis. We have previously reported that HIV-1 Nef is responsible for the induction of a severe AIDS-like disease in CD4C/HIV transgenic (Tg) mice. To understand the molecular mechanisms of this Nef-induced disease, we generated Tg mice expressing a mutated Nef protein in which the SH3 ligand-binding domain (P(72)XXP(75)XXP(78)) was mutated to A(72)XXA(75)XXQ(78). This mutation completely abolished the pathogenic potential of Nef, although a partial downregulation of the CD4 cell surface expression was still observed in these Tg mice. We also studied whether Hck, one of the effectors previously found to bind to this PXXP motif of Nef, was involved in disease development. Breeding of Tg mice expressing wild-type Nef on an hck(-/-) (knockout) background did not abolish any of the pathological phenotypes. However, the latency of disease development was prolonged. These data indicate that an intact PXXP domain is essential for inducing an AIDS-like disease in CD4C/HIV Tg mice and suggest that interaction of a cellular effector(s) with this domain is required for the induction of this multiorgan disease. Our findings indicate that Hck is an important, but not an essential, effector of Nef and suggest that another factor(s), yet to be identified, may be more critical for disease development.

MeSH Terms
Animals CD4 Antigens/genetics Genes, nef HIV Infections/genetics,virology HIV-1/genetics,pathogenicity Humans Mice Mice, Transgenic Mutation Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-hck Virulence/genetics Virus Replication/genetics src Homology Domains
Chemicals
CD4 Antigens Proto-Oncogene Proteins Protein-Tyrosine Kinases HCK protein, human Hck protein, mouse Proto-Oncogene Proteins c-hck
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hanna Z
Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada. hannaz@ircm.qc.ca
Weng X
Kay D G
Poudrier J
Lowell C
Jolicoeur P
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
2001-10-00
Pages
9378-92
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC114506
Subset
IM
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