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PMID: 11532950 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Improvement of reading frame maintenance is a common function for several tRNA modifications.

The EMBO journal ·Vol. 20 ·No. 17 ·2001-09-03 ·Pages 4863-73

Urbonavicius J, Qian Q, Durand JM, Hagervall TG, Björk GR

Abstract

Transfer RNAs from all organisms contain many modified nucleosides. Their vastly different chemical structures, their presence in different tRNAs, their occurrence in different locations in tRNA and their influence on different reactions in which tRNA participates suggest that each modified nucleoside may have its own specific function. However, since the frequency of frameshifting in several different mutants [mnmA, mnmE, tgt, truA (hisT), trmD, miaA, miaB and miaE] defective in tRNA modification was higher compared with the corresponding wild-type controls, these modifications have a common function: they all improve reading frame maintenance. Frameshifting occurs by peptidyl-tRNA slippage, which is influenced by the hypomodified tRNA in two ways: (i) a hypomodified tRNA in the ternary complex may decrease the rate by which the complex is recruited to the A-site and thereby increasing peptidyl-tRNA slippage; or (ii) a hypomodified peptidyl-tRNA may be more prone to slip than its fully modified counterpart. We propose that the improvement of reading frame maintenance has been and is the major selective factor for the emergence of new modified nucleosides.

MeSH Terms
Base Sequence Codon/genetics Escherichia coli/genetics Frameshift Mutation Genotype Models, Genetic Oligodeoxyribonucleotides/chemistry Phenotype RNA, Bacterial/genetics RNA, Transfer/chemistry,genetics RNA, Transfer, Amino Acid-Specific/genetics RNA, Transfer, Amino Acyl/chemistry,genetics RNA, Transfer, Leu/genetics RNA, Transfer, Phe/genetics RNA, Transfer, Pro/genetics RNA, Transfer, Val/genetics Reading Frames Reference Values Salmonella typhimurium/genetics beta-Galactosidase/genetics beta-Lactamases/genetics
Chemicals
Codon Oligodeoxyribonucleotides RNA, Bacterial RNA, Transfer, Amino Acid-Specific RNA, Transfer, Amino Acyl RNA, Transfer, Leu RNA, Transfer, Phe RNA, Transfer, Pro RNA, Transfer, Val tRNA, peptidyl- RNA, Transfer beta-Galactosidase beta-Lactamases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Urbonavicius J
Department of Microbiology, Umeå University, S-90 187 Umeå, Sweden.
Qian Q
Durand J M
Hagervall T G
Björk G R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.17.4863
Published
2001-09-03
Pages
4863-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125605
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.17.4863 PMC Full Text View journal details
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