Immunocyte Ca2+ influx system mediated by LTRPC2.

Science (New York, N.Y.) ·Vol. 293 ·No. 5533 ·2001-08-17 ·Pages 1327-30

Sano Y, Inamura K, Miyake A, Mochizuki S, Yokoi H, Matsushime H, Furuichi K

Abstract

We characterized an activation mechanism of the human LTRPC2 protein, a member of the transient receptor potential family of ion channels, and demonstrated that LTRPC2 mediates Ca2+ influx into immunocytes. Intracellular pyrimidine nucleotides, adenosine 5'-diphosphoribose (ADPR), and nicotinamide adenine dinucleotide (NAD), directly activated LTRPC2, which functioned as a Ca2+-permeable nonselective cation channel and enabled Ca2+ influx into cells. This activation was suppressed by intracellular adenosine triphosphate. These results reveal that ADPR and NAD act as intracellular messengers and may have an important role in Ca2+ influx by activating LTRPC2 in immunocytes.

MeSH Terms

ADP-ribosyl Cyclase ADP-ribosyl Cyclase 1 Adenosine Diphosphate Ribose/metabolism,pharmacology Adenosine Triphosphate/metabolism,pharmacology Antigens, CD Antigens, Differentiation/metabolism Apoptosis Calcium/metabolism Calcium Channels/metabolism Cell Line Eosinophils/metabolism Humans Ion Channels Jurkat Cells Membrane Glycoproteins Membrane Potentials Membrane Proteins Monocytes/metabolism NAD/metabolism,pharmacology NAD+ Nucleosidase/metabolism Patch-Clamp Techniques Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes/metabolism TRPM Cation Channels

Chemicals

Antigens, CD Antigens, Differentiation Calcium Channels Ion Channels Membrane Glycoproteins Membrane Proteins TRPM Cation Channels TRPM2 protein, human NAD Adenosine Diphosphate Ribose Adenosine Triphosphate ADP-ribosyl Cyclase CD38 protein, human NAD+ Nucleosidase ADP-ribosyl Cyclase 1 Calcium

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Sano Y

Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Tsukuba, Ibaraki 305-8585, Japan. sano.yorikata@yamanouchi.co.jp

Inamura K

Miyake A

Mochizuki S

Yokoi H

Matsushime H

Furuichi K

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.1062473

sano.yorikata@yamanouchi.co.jp

Published

2001-08-17

Pages

1327-30

Language

English

Region

United States

NLM ID

0404511

Subset

Corrections

CommentIn

PMID: 11509717

External Links

PubMed source DOI: 10.1126/science.1062473 View journal details

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Immunocyte Ca2+ influx system mediated by LTRPC2.

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