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PMID: 11509180 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of CSF1 promoter by the SWI/SNF-like BAF complex.

Cell ·Vol. 106 ·No. 3 ·2001-08-10 ·Pages 309-18

Liu R, Liu H, Chen X, Kirby M, Brown PO, Zhao K

Abstract

The mammalian BAF complex regulates gene expression by modifying chromatin structure. In this report, we identify 80 genes activated and 2 genes repressed by the BAF complex in SW-13 cells. We find that prior binding of NFI/CTF to the NFI/CTF binding site in CSF1 promoter is required for the recruitment of the BAF complex and the BAF-dependent activation of the promoter. Furthermore, the activation of the CSF1 promoter requires Z-DNA-forming sequences that are converted to Z-DNA structure upon activation by the BAF complex. The BAF complex facilitates Z-DNA formation in a nucleosomal template in vitro. We propose a model in which the BAF complex promotes Z-DNA formation which, in turn, stabilizes the open chromatin structure at the CSF1 promoter.

MeSH Terms

Base Sequence CCAAT-Enhancer-Binding Proteins/metabolism Chromatin/chemistry,genetics,metabolism DNA Helicases DNA, Superhelical/chemistry,genetics,metabolism DNA-Binding Proteins/chemistry,metabolism Gene Expression Profiling Gene Expression Regulation Gene Silencing Humans Macromolecular Substances Macrophage Colony-Stimulating Factor/genetics Models, Genetic Molecular Sequence Data NFI Transcription Factors Nuclear Proteins/genetics,metabolism Nucleic Acid Conformation Oligonucleotide Array Sequence Analysis Plasmids/chemistry,genetics,metabolism Promoter Regions, Genetic/genetics Protein Binding Protein Subunits RNA, Messenger/genetics,metabolism Response Elements/genetics Templates, Genetic Transcription Factors/chemistry,genetics,metabolism Transcriptional Activation Tumor Cells, Cultured

Chemicals

CCAAT-Enhancer-Binding Proteins CTF-1 transcription factor Chromatin DNA, Superhelical DNA-Binding Proteins Macromolecular Substances NFI Transcription Factors Nuclear Proteins Protein Subunits RNA, Messenger SMARCA2 protein, human Transcription Factors Macrophage Colony-Stimulating Factor SMARCA4 protein, human DNA Helicases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Liu R

Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Liu H

Chen X

Kirby M

Brown P O

Zhao K

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(01)00446-9

Published

2001-08-10

Pages

309-18

Language

English

Region

United States

NLM ID

0413066

Subset

External Links

PubMed source DOI: 10.1016/s0092-8674(01)00446-9 View journal details

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Regulation of CSF1 promoter by the SWI/SNF-like BAF complex.

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