Peptides corresponding to the heptad repeat motifs in the transmembrane protein (gp41) of human immunodeficiency virus type 1 elicit antibodies to receptor-activated conformations of the envelope glycoprotein.

Journal of virology ·Vol. 75 ·No. 18 ·2001-09-00 ·Pages 8859-63

de Rosny E, Vassell R, Wingfield PT, Wild CT, Weiss CD

Abstract

Two heptad repeat regions in the ectodomain of the human immunodeficiency virus type 1 (HIV-1) transmembrane subunit (gp41) self-assemble into a six-helix bundle structure that is critical for virus entry. Immunizations with peptides corresponding to these regions generated antibodies specific to the receptor-activated conformations of gp41.

MeSH Terms

Amino Acid Sequence Animals CD4 Antigens/immunology Cell Line, Transformed HIV Antibodies/immunology HIV Envelope Protein gp41/chemistry,immunology HIV-1/immunology Humans Molecular Sequence Data Peptides/chemistry,immunology Protein Conformation Rabbits Repetitive Sequences, Nucleic Acid

Chemicals

CD4 Antigens HIV Antibodies HIV Envelope Protein gp41 Peptides

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

de Rosny E

Office of Vaccines, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Bethesda, Maryland 20892-4555, USA.

Vassell R

Wingfield P T

Wild C T

Weiss C D

References (18)

18 references, click to expand

Recognition by human monoclonal antibodies of free and complexed peptides representing the prefusogenic and fusogenic forms of human immunodeficiency virus type 1 gp41.
J Virol. 2000 Jul;74(13):6186-92 PMID: 10846104
A conformation-specific monoclonal antibody reacting with fusion-active gp41 from the human immunodeficiency virus type 1 envelope glycoprotein.
J Virol. 1998 Dec;72(12):10213-7 PMID: 9811763
Detection of antibodies to human T-lymphotropic virus type III by using a synthetic peptide of 21 amino acid residues corresponding to a highly antigenic segment of gp41 envelope protein.
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6159-63 PMID: 2426711
Biosynthesis, cleavage, and degradation of the human immunodeficiency virus 1 envelope glycoprotein gp160.
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9580-4 PMID: 2849111
A synthetic peptide inhibitor of human immunodeficiency virus replication: correlation between solution structure and viral inhibition.
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10537-41 PMID: 1438243
HIV-1 inhibition by a peptide.
Nature. 1993 Sep 9;365(6442):113 PMID: 8371754
A conserved neutralizing epitope on gp41 of human immunodeficiency virus type 1.
J Virol. 1993 Nov;67(11):6642-7 PMID: 7692082
Distinct modes of human immunodeficiency virus type 1 proviral latency revealed by superinfection of nonproductively infected cell lines with recombinant luciferase-encoding viruses.
J Virol. 1994 Feb;68(2):654-60 PMID: 7507183
Peptides corresponding to a predictive alpha-helical domain of human immunodeficiency virus type 1 gp41 are potent inhibitors of virus infection.
Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9770-4 PMID: 7937889
A trimeric structural domain of the HIV-1 transmembrane glycoprotein.
Nat Struct Biol. 1995 Dec;2(12):1075-82 PMID: 8846219
Core structure of gp41 from the HIV envelope glycoprotein.
Cell. 1997 Apr 18;89(2):263-73 PMID: 9108481
Atomic structure of the ectodomain from HIV-1 gp41.
Nature. 1997 May 22;387(6631):426-30 PMID: 9163431
The extracellular domain of immunodeficiency virus gp41 protein: expression in Escherichia coli, purification, and crystallization.
Protein Sci. 1997 Aug;6(8):1653-60 PMID: 9260278
Atomic structure of a thermostable subdomain of HIV-1 gp41.
Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12303-8 PMID: 9356444
Capture of an early fusion-active conformation of HIV-1 gp41.
Nat Struct Biol. 1998 Apr;5(4):276-9 PMID: 9546217
HIV entry and its inhibition.
Cell. 1998 May 29;93(5):681-4 PMID: 9630213
Mutational analysis of residues in the coiled-coil domain of human immunodeficiency virus type 1 transmembrane protein gp41.
J Virol. 1998 Dec;72(12):9676-82 PMID: 9811701
Evidence that the transition of HIV-1 gp41 into a six-helix bundle, not the bundle configuration, induces membrane fusion.
J Cell Biol. 2000 Oct 16;151(2):413-23 PMID: 11038187

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/jvi.75.18.8859-8863.2001

Published

2001-09-00

Pages

8859-63

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC115132

Subset

External Links

PubMed source DOI: 10.1128/jvi.75.18.8859-8863.2001 PMC Full Text View journal details

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Peptides corresponding to the heptad repeat motifs in the transmembrane protein (gp41) of human immunodeficiency virus type 1 elicit antibodies to receptor-activated conformations of the envelope glycoprotein.

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