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PMID: 11500568 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A Medicago truncatula homoglutathione synthetase is derived from glutathione synthetase by gene duplication.

Plant physiology ·Vol. 126 ·No. 4 ·2001-08-00 ·Pages 1706-15

Frendo P, Jiménez MJ, Mathieu C, Duret L, Gallesi D, Van de Sype G, Hérouart D, Puppo A

Abstract

Glutathione (GSH) and homo-GSH (hGSH) are the major low-molecular weight thiols synthesized in Medicago truncatula. Two M. truncatula cDNAs (gshs1 and gshs2) corresponding to a putative GSH synthetase (GSHS) and a putative hGSH synthetase (hGSHS) were characterized. Heterologous expression of gshs1 and gshs2 cDNAs in an Escherichia coli strain deficient in GSHS activity showed that GSHS1 and GSHS2 are a GSHS and an hGSHS, respectively. Leucine-534 and proline-535 present in hGSHS were substituted by alanines that are conserved in plant GSHS. These substitutions resulted in a strongly stimulated GSH accumulation in the transformed E. coli strain showing that these residues play a crucial role in the differential recognition of beta-alanine and glycine by hGSHS. Phylogenetic analysis of GSHS2 and GSHS1 with other eukaryotic GSHS sequences indicated that gshs2 and gshs1 are the result of a gene duplication that occurred after the divergence between Fabales, Solanales, and Brassicales. Analysis of the structure of gshs1 and gshs2 genes shows they are both present in a cluster and in the same orientation in the M. truncatula genome, suggesting that the duplication of gshs1 and gshs2 occurred via a tandem duplication.

MeSH Terms
Amino Acid Sequence Animals Chromosome Mapping DNA, Complementary/analysis DNA, Plant/analysis Escherichia coli/genetics Gene Duplication Genes, Plant Glutathione Synthase/classification,genetics,metabolism Humans Medicago sativa/enzymology,genetics Molecular Sequence Data Peptide Synthases/classification,genetics,metabolism Phylogeny Sequence Homology, Amino Acid Tandem Repeat Sequences
Chemicals
DNA, Complementary DNA, Plant Peptide Synthases homoglutathione synthase Glutathione Synthase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Frendo P
Laboratoire de Biologie Végétale et Microbiologie, Centre National de la Recherche Scientifique, Formation de Recherche en Evolution 2294, Université de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice cedex 2, France. frendo@unice.fr
Jiménez M J
Mathieu C
Duret L
Gallesi D
Van de Sype G
Hérouart D
Puppo A
References (21)
21 references, click to expand
  1. ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites.
    Protein Sci. 1999 May;8(5):978-84 PMID: 10338008
  2. The neighbor-joining method: a new method for reconstructing phylogenetic trees.
    Mol Biol Evol. 1987 Jul;4(4):406-25 PMID: 3447015
  3. A new thiol in legumes.
    Nature. 1957 Jul 20;180(4577):148-9 PMID: 13451668
  4. Glutathione synthetase. Purification from rat kidney and mapping of the substrate binding sites.
    J Biol Chem. 1979 Jun 25;254(12):5184-90 PMID: 447639
  5. Enzymatic reactions of ascorbate and glutathione that prevent peroxide damage in soybean root nodules.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3811-5 PMID: 16593704
  6. Tripeptide (glutathione) synthetase. Purification, properties, and mechanism of action.
    Biochemistry. 1967 Jun;6(6):1722-34 PMID: 6035915
  7. STRUCTURE AND PROPERTIES OF A HOMOLOGUE OF GLUTATHIONE.
    Biochem J. 1963 Dec;89:471-8 PMID: 14101966
  8. gamma-Glutamylcysteinylglutamic acid--a new homologue of glutathione in maize seedlings exposed to cadmium.
    FEBS Lett. 1993 Dec 28;336(3):472-6 PMID: 8282113
  9. Glutathione and homoglutathione synthesis in legume root nodules.
    Plant Physiol. 1999 Nov;121(3):879-88 PMID: 10557236
  10. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  11. Synthesis of Phytochelatins and Homo-Phytochelatins in Pisum sativum L.
    Plant Physiol. 1995 Feb;107(2):515-521 PMID: 12228379
  12. Glutathione and homoglutathione synthetases of legume nodules. Cloning, expression, and subcellular localization.
    Plant Physiol. 2000 Nov;124(3):1381-92 PMID: 11080313
  13. A knowledge base for predicting protein localization sites in eukaryotic cells.
    Genomics. 1992 Dec;14(4):897-911 PMID: 1478671
  14. Purification and properties of glutathione synthetase from bovine lens.
    Exp Eye Res. 1977 Feb;24(2):145-58 PMID: 14840
  15. The gene encoding human glutathione synthetase (GSS) maps to the long arm of chromosome 20 at band 11.2.
    Genomics. 1995 Dec 10;30(3):617-9 PMID: 8825653
  16. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  17. An efficient method for isolation of RNA from tissue cultured plant cells.
    Nucleic Acids Res. 1991 Nov 11;19(21):6055 PMID: 1719489
  18. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  19. Molecular basis of glutathione synthetase deficiency and a rare gene permutation event.
    EMBO J. 1999 Jun 15;18(12):3204-13 PMID: 10369661
  20. Substrate and thiol specificity of a stress-inducible glutathione transferase from soybean.
    FEBS Lett. 1997 Jun 16;409(3):370-4 PMID: 9224692
  21. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.126.4.1706
Email
frendo@unice.fr
Published
2001-08-00
Pages
1706-15
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC117169
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.126.4.1706 PMC Full Text View journal details
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