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PMID: 11489848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A program of Yersinia enterocolitica type III secretion reactions is activated by specific signals.

Journal of bacteriology ·Vol. 183 ·No. 17 ·2001-09-00 ·Pages 4970-8

Lee VT, Mazmanian SK, Schneewind O

Abstract

Successful establishment of Yersinia infections requires the type III machinery, a protein transporter that injects virulence factors (Yops) into macrophages. It is reported here that the Yersinia type III pathway responds to environmental signals by transporting proteins to distinct locations. Yersinia enterocolitica cells sense an increase in extracellular amino acids (glutamate, glutamine, aspartate, and asparagine) that results in the activation of the type III pathway. Another signal, provided by serum proteins such as albumin, triggers the secretion of YopD into the extracellular medium. The third signal, a decrease in calcium concentration, appears to be provided by host cells and causes Y. enterocolitica to transport YopE and presumably other virulence factors across the eukaryotic plasma membrane. Mutations in several genes encoding regulatory molecules (lcrG, lcrH, tyeA, yopD, yopN, yscM1, and yscM2) bypass the signal requirement of the type III pathway. Together these results suggest that yersiniae may have evolved distinct secretion reactions in response to environmental signals.

MeSH Terms
Albumins/metabolism Asparagine/metabolism Aspartic Acid/metabolism Bacterial Outer Membrane Proteins/metabolism Calcium/metabolism Genes, Regulator/genetics Glutamic Acid/metabolism Glutamine/metabolism HeLa Cells Humans Mutation Signal Transduction/genetics,physiology Yersinia enterocolitica/physiology
Chemicals
Albumins Bacterial Outer Membrane Proteins YopD protein, Yersinia yopE protein, Yersinia Glutamine Aspartic Acid Glutamic Acid Asparagine Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee V T
Department of Microbiology and Immunology, University of California Los Angeles School of Medicine, Los Angeles, California 90095, USA.
Mazmanian S K
Schneewind O
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.17.4970-4978.2001
Published
2001-09-00
Pages
4970-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95371
Subset
IM
Grants
NIAID NIH HHS · R01 AI042797 · United States
NIGMS NIH HHS · T32 GM007104 · United States
NIAID NIH HHS · AI42797 · United States
NIGMS NIH HHS · GM07104 · United States
External Links
PubMed source DOI: 10.1128/JB.183.17.4970-4978.2001 PMC Full Text View journal details
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