Home LiteratureArticle Details
PMID: 11487634 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Spine formation and correlated assembly of presynaptic and postsynaptic molecules.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 21 ·No. 16 ·2001-08-15 ·Pages 6105-14

Okabe S, Miwa A, Okado H

Abstract

Hippocampal pyramidal neurons in culture showed a developmental shift in synapse distribution from dendritic shafts to spines. Using dual wavelength time-lapse fluorescence microscopy, we analyzed the morphogenesis of three synaptic components: dendritic spines, postsynaptic densities (PSDs), and presynaptic vesicles. Local assembly of a major PSD protein, PSD-95, was spatially and temporally correlated with spine morphogenesis. Clustering of postsynaptic PSD-95 and that of a predominant synaptic vesicle protein, synaptophysin, were also correlated. In contrast, pre-existing PSD-95 clusters in dendritic shafts were preferentially eliminated without promoting spine formation. The local and stepwise assembly of synaptic components at the contact sites between dendritic protrusions and axons explains the developmental remodeling of excitatory synapses.

MeSH Terms
Adenoviridae/genetics Aging/metabolism Animals Axons/metabolism,ultrastructure Cell Differentiation Cell Surface Extensions/metabolism,ultrastructure Cells, Cultured Dendrites/metabolism,ultrastructure Disks Large Homolog 4 Protein Genes, Reporter Guanylate Kinases Hippocampus Immunohistochemistry Intracellular Signaling Peptides and Proteins Luminescent Proteins/genetics Membrane Proteins Mice Microscopy, Fluorescence Nerve Tissue Proteins/genetics,metabolism Protein Transport/physiology Pseudopodia/metabolism,ultrastructure Pyramidal Cells/metabolism,ultrastructure Rats Recombinant Fusion Proteins/genetics,metabolism Synapses/metabolism,ultrastructure Synaptic Vesicles/metabolism,ultrastructure Synaptophysin/metabolism Transfection
Chemicals
Disks Large Homolog 4 Protein Dlg4 protein, mouse Dlg4 protein, rat Intracellular Signaling Peptides and Proteins Luminescent Proteins Membrane Proteins Nerve Tissue Proteins Recombinant Fusion Proteins Synaptophysin postsynaptic density proteins Guanylate Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Okabe S
Department of Anatomy and Cell Biology, School of Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, 113-8519, Japan. okabe.cbio@tmd.ac.jp
Miwa A
Okado H
References (35)
35 references, click to expand
  1. Experience-dependent plasticity of dendritic spines in the developing rat barrel cortex in vivo.
    Nature. 2000 Apr 20;404(6780):876-81 PMID: 10786794
  2. Evidence for a role of dendritic filopodia in synaptogenesis and spine formation.
    Neuron. 1996 Jul;17(1):91-102 PMID: 8755481
  3. The development of excitatory synapses in cultured spinal neurons.
    J Neurosci. 1997 Oct 1;17(19):7339-50 PMID: 9295380
  4. Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.
    Proc Natl Acad Sci U S A. 1996 Feb 6;93(3):1320-4 PMID: 8577762
  5. Synapsin I in nerve terminals: selective association with small synaptic vesicles.
    Science. 1984 Dec 7;226(4679):1209-11 PMID: 6438799
  6. Dendritic spine changes associated with hippocampal long-term synaptic plasticity.
    Nature. 1999 May 6;399(6731):66-70 PMID: 10331391
  7. Organization and regulation of proteins at synapses.
    Curr Opin Cell Biol. 1999 Apr;11(2):248-54 PMID: 10209161
  8. Alternative splicing of the C-terminal domain regulates cell surface expression of the NMDA receptor NR1 subunit.
    J Neurosci. 1999 Sep 15;19(18):7781-92 PMID: 10479681
  9. SAP90, a rat presynaptic protein related to the product of the Drosophila tumor suppressor gene dlg-A.
    J Biol Chem. 1993 Mar 5;268(7):4580-3 PMID: 7680343
  10. Efficient selection for high-expression transfectants with a novel eukaryotic vector.
    Gene. 1991 Dec 15;108(2):193-9 PMID: 1660837
  11. Heterogeneity in the molecular composition of excitatory postsynaptic sites during development of hippocampal neurons in culture.
    J Neurosci. 1998 Feb 15;18(4):1217-29 PMID: 9454832
  12. A simple and efficient method for purification of infectious recombinant adenovirus.
    Jpn J Med Sci Biol. 1994 Jun;47(3):157-66 PMID: 7823411
  13. PSD-95 involvement in maturation of excitatory synapses.
    Science. 2000 Nov 17;290(5495):1364-8 PMID: 11082065
  14. Glutamate receptor anchoring proteins and the molecular organization of excitatory synapses.
    Ann N Y Acad Sci. 1999 Apr 30;868:483-93 PMID: 10414325
  15. LTP promotes formation of multiple spine synapses between a single axon terminal and a dendrite.
    Nature. 1999 Nov 25;402(6760):421-5 PMID: 10586883
  16. Synaptic vesicle dynamics in living cultured hippocampal neurons visualized with CY3-conjugated antibodies directed against the lumenal domain of synaptotagmin.
    J Neurosci. 1995 Jun;15(6):4328-42 PMID: 7540672
  17. Rapid dendritic morphogenesis in CA1 hippocampal dendrites induced by synaptic activity.
    Science. 1999 Mar 19;283(5409):1923-7 PMID: 10082466
  18. Activity regulates the synaptic localization of the NMDA receptor in hippocampal neurons.
    Neuron. 1997 Oct;19(4):801-12 PMID: 9354327
  19. The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein.
    Neuron. 1992 Nov;9(5):929-42 PMID: 1419001
  20. Signal transduction molecules at the glutamatergic postsynaptic membrane.
    Brain Res Brain Res Rev. 1998 May;26(2-3):243-57 PMID: 9651538
  21. Survival and synaptogenesis of hippocampal neurons without NMDA receptor function in culture.
    Eur J Neurosci. 1998 Jun;10(6):2192-8 PMID: 9753105
  22. Continual remodeling of postsynaptic density and its regulation by synaptic activity.
    Nat Neurosci. 1999 Sep;2(9):804-11 PMID: 10461219
  23. Assembly of presynaptic active zones from cytoplasmic transport packets.
    Nat Neurosci. 2000 May;3(5):445-51 PMID: 10769383
  24. Visualization of the dynamics of synaptic vesicle and plasma membrane proteins in living axons.
    J Cell Biol. 1998 Feb 9;140(3):659-74 PMID: 9456325
  25. Assembly of new individual excitatory synapses: time course and temporal order of synaptic molecule recruitment.
    Neuron. 2000 Jul;27(1):57-69 PMID: 10939331
  26. Fine structure of neurons and synapses in the feline hippocampus during postnatal ontogenesis.
    Exp Neurol. 1968 Nov;22(3):394-407 PMID: 5707004
  27. Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA.
    EMBO J. 1987 Nov;6(11):3261-8 PMID: 3123215
  28. Three-dimensional structure of dendritic spines and synapses in rat hippocampus (CA1) at postnatal day 15 and adult ages: implications for the maturation of synaptic physiology and long-term potentiation.
    J Neurosci. 1992 Jul;12(7):2685-705 PMID: 1613552
  29. Neuroligin expressed in nonneuronal cells triggers presynaptic development in contacting axons.
    Cell. 2000 Jun 9;101(6):657-69 PMID: 10892652
  30. Synaptogenesis via dendritic filopodia in developing hippocampal area CA1.
    J Neurosci. 1998 Nov 1;18(21):8900-11 PMID: 9786995
  31. The distribution of synapsin I and synaptophysin in hippocampal neurons developing in culture.
    J Neurosci. 1991 Jun;11(6):1617-26 PMID: 1904480
  32. The dynamics of dendritic structure in developing hippocampal slices.
    J Neurosci. 1996 May 1;16(9):2983-94 PMID: 8622128
  33. Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.
    Science. 1995 Sep 22;269(5231):1737-40 PMID: 7569905
  34. Morphological analysis of dendritic spine development in primary cultures of hippocampal neurons.
    J Neurosci. 1995 Jan;15(1 Pt 1):1-11 PMID: 7823120
  35. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.
    Nucleic Acids Res. 1995 Oct 11;23(19):3816-21 PMID: 7479022
Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Email
okabe.cbio@tmd.ac.jp
Published
2001-08-15
Pages
6105-14
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6763142
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2