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PMID: 1147905 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Optical measurement of the catalase-hydrogen peroxide intermediate (Compound I) in the liver of anaesthetized rats and its implication to hydrogen peroxide production in situ.

The Biochemical journal ·Vol. 146 ·No. 1 ·1975-01-00 ·Pages 67-77

Oshino N, Jamieson D, Sugano T, Chance B

Abstract

The spectrophotometric determination of the catalase-H2O2 intermediate (Compound I) was extended to the liver in situ in anaesthetized rats. The rate of H2O2 production was determined for the liver in situ with endogenous substrates, and in the presence of excess of glycollate. Glycollate infusion doubled H2O2 production rate in the liver of air-breathing rats, and caused a fourfold increase when rats breathed O2 at 1 times 10(5) Pa. Hyperbaric O2 up to 6 times 10(5) Pa did not increase H2O2 generation supported by endogenous substrates, nor did it increase H2O2 production above that produced by 1 times 10(5) Pa O2 in glycollate-supplemented rats. The rates of ethanol oxidation via hepatic catalase and via alcohol dehydrogenase in the whole body were separately measured. The contribution of hepatic catalase to ethanol oxidation was found to be approx. 10 percent in endogenous conditions and increased to 30 percent or more of the total ethanol oxidation in rats supplemented with glycolate.

MeSH Terms
Alcohol Oxidoreductases/metabolism Anesthesia, General Animals Catalase/analysis Ethanol/blood,metabolism Glycolates/metabolism Hydrogen Peroxide/analysis,biosynthesis Hyperbaric Oxygenation Liver/drug effects,enzymology,metabolism Male Methanol/metabolism Oxidation-Reduction Oxygen Consumption Pyrazoles/pharmacology Rats Spectrophotometry Triazoles/pharmacology
Chemicals
Glycolates Pyrazoles Triazoles Ethanol Hydrogen Peroxide Alcohol Oxidoreductases Catalase Methanol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oshino N
Jamieson D
Sugano T
Chance B
References (30)
30 references, click to expand
  1. Kinetics of mitochondrial flavoprotein and pyridine nucleotide in perfused heart.
    Am J Physiol. 1972 Jul;223(1):207-18 PMID: 4339003
  2. The specificities and configurations of ternary complexes of yeast and liver alcohol dehydrogenases.
    Biochem J. 1967 Jul;104(1):165-72 PMID: 4291993
  3. Superoxide radicals as precursors of mitochondrial hydrogen peroxide.
    FEBS Lett. 1974 May 15;42(1):68-72 PMID: 4859511
  4. The properties of hydrogen peroxide production under hyperoxic and hypoxic conditions of perfused rat liver.
    Biochem J. 1975 Jan;146(1):53-65 PMID: 167718
  5. The role of H 2 O 2 generation in perfused rat liver and the reaction of catalase compound I and hydrogen donors.
    Arch Biochem Biophys. 1973 Jan;154(1):117-31 PMID: 4347674
  6. Analysis of the catalase--hydrogen peroxide intermediate in coupled oxidations.
    Biochem J. 1973 Mar;131(3):564-7 PMID: 4720714
  7. State of oxidation-reduction and state of binding in the cytosolic NADH-system as disclosed by equilibration with extracellular lactate-pyruvate in hemoglobin-free perfused rat liver.
    Eur J Biochem. 1972 May 23;27(2):301-17 PMID: 4340564
  8. Evidence that peroxidation of lysosomal membranes is initiated by hydroxyl free radicals produced during flavin enzyme activity.
    J Biol Chem. 1973 Nov 25;248(22):7792-7 PMID: 4147983
  9. Seizures, H 2 O 2 formation and lipid peroxides in brain during exposure to oxygen under high pressure.
    Aerosp Med. 1973 Jan;44(1):40-4 PMID: 4689483
  10. Spectrophotometric studies on intact muscle. I. Components of the respiratory chain.
    J Gen Physiol. 1963 May;46:905-28 PMID: 14041948
  11. Regulation of the pentose phosphate cycle.
    Biochem J. 1974 Mar;138(3):425-35 PMID: 4154743
  12. In vivo formation of H2O2 in red cells during exposure to hyperoxia.
    J Clin Invest. 1972 Aug;51(8):2211-3 PMID: 5054473
  13. Kinetics and mechanisms of catalase in peroxisomes of the mitochondrial fraction.
    Biochem J. 1971 Apr;122(2):225-33 PMID: 5117568
  14. The metabolism of ethanol and its metabolic effects.
    Pharmacol Rev. 1972 Mar;24(1):67-157 PMID: 4402043
  15. The kinetics and mechanism of liver alcohol dehydrogenase with primary and secondary alcohols as substrates.
    Biochem J. 1966 Jul;100(1):34-46 PMID: 4290533
  16. Oxidation-reduction changes of cytochromes following stimulation of amphibian cardiac muscle.
    J Physiol. 1959 Jun 23;147(1):14-32 PMID: 13673381
  17. Role of the intracellular distribution of hepatic catalase in the peroxidative oxidation of methanol.
    Ann N Y Acad Sci. 1969 Dec 19;168(2):265-80 PMID: 4988806
  18. The combustion of alcohol and its inhibition by 4-methyl-pyrazole in perfused rat livers.
    Arch Biochem Biophys. 1972 Aug;151(2):434-44 PMID: 4339931
  19. The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen.
    Biochem J. 1973 Jul;134(3):707-16 PMID: 4749271
  20. Metabolic alterations produced in the liver by chronic ethanol administration. Increased oxidative capacity.
    Biochem J. 1973 Jun;134(2):507-14 PMID: 16742811
  21. Heme occupancy of catalase in hemoglobin-free perfused rat liver and of isolated rat liver catalase.
    Arch Biochem Biophys. 1973 Jan;154(1):106-16 PMID: 4689773
  22. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).
    J Biol Chem. 1969 Nov 25;244(22):6049-55 PMID: 5389100
  23. Utilization and metabolic effects of acetaldehyde and ethanol in the perfused rat liver.
    Biochem J. 1972 Feb;126(4):945-52 PMID: 4342167
  24. The acceptor specificity of flavins and flavoproteins. 3. Flavoproteins.
    Biochim Biophys Acta. 1971 Mar 2;226(2):269-84 PMID: 4396857
  25. Metabolic effects of epinephrine in the perfused rat heart. I. Comparison of intracellular redox states, tissue pO2 and force of contraction.
    Mol Pharmacol. 1966 May;2(3):191-205 PMID: 5962064
  26. The steady state level of catalase compound I in isolated hemoglobin-free perfused rat liver.
    FEBS Lett. 1970 Dec;11(3):172-176 PMID: 11945479
  27. Spectrophotometric studies on intact muscle. II. Recovery from contractile activity.
    J Gen Physiol. 1963 May;46:929-69 PMID: 13957780
  28. The kinetics of catalase synthesis and destruction in vivo.
    J Biol Chem. 1962 Nov;237:3468-75 PMID: 13986151
  29. The characteristics of the "peroxidatic" reaction of catalase in ethanol oxidation.
    Biochem J. 1973 Mar;131(3):555-63 PMID: 4720713
  30. On the inhibitory power of some further pyrazole derivatives of horse liver alcohol dehydrogenase.
    Biochem Biophys Res Commun. 1974 Apr 8;57(3):549-53 PMID: 4827822
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1460067
Published
1975-01-00
Pages
67-77
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165276
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1460067 PMC Full Text View journal details
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