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PMID: 11459974 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Roles of DNA polymerases V and II in SOS-induced error-prone and error-free repair in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 15 ·2001-07-17 ·Pages 8350-4

Pham P, Rangarajan S, Woodgate R, Goodman MF

Abstract

DNA polymerase V, composed of a heterotrimer of the DNA damage-inducible UmuC and UmuD(2)(') proteins, working in conjunction with RecA, single-stranded DNA (ssDNA)-binding protein (SSB), beta sliding clamp, and gamma clamp loading complex, are responsible for most SOS lesion-targeted mutations in Escherichia coli, by catalyzing translesion synthesis (TLS). DNA polymerase II, the product of the damage-inducible polB (dinA ) gene plays a pivotal role in replication-restart, a process that bypasses DNA damage in an error-free manner. Replication-restart takes place almost immediately after the DNA is damaged (approximately 2 min post-UV irradiation), whereas TLS occurs after pol V is induced approximately 50 min later. We discuss recent data for pol V-catalyzed TLS and pol II-catalyzed replication-restart. Specific roles during TLS for pol V and each of its accessory factors have been recently determined. Although the precise molecular mechanism of pol II-dependent replication-restart remains to be elucidated, it has recently been shown to operate in conjunction with RecFOR and PriA proteins.

MeSH Terms
Catalysis DNA Polymerase II/metabolism,physiology DNA Repair DNA, Bacterial/biosynthesis DNA-Directed DNA Polymerase/metabolism,physiology Escherichia coli/enzymology,genetics Escherichia coli Proteins Models, Genetic Mutagenesis Rec A Recombinases/metabolism SOS Response, Genetics
Chemicals
DNA, Bacterial Escherichia coli Proteins Rec A Recombinases DNA Polymerase II DNA polymerase V, E coli DNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pham P
Department of Biological Sciences, Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles, CA 90089-1340, USA.
Rangarajan S
Woodgate R
Goodman M F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.111007198
Published
2001-07-17
Pages
8350-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC37442
Subset
IM
Grants
NIGMS NIH HHS · R01 GM021422 · United States
NIGMS NIH HHS · R37 GM021422 · United States
NIGMS NIH HHS · GM21422 · United States
NIGMS NIH HHS · GM42554 · United States
External Links
PubMed source DOI: 10.1073/pnas.111007198 PMC Full Text View journal details
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