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PMID: 11459939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

M cell-targeted DNA vaccination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 16 ·2001-07-31 ·Pages 9318-23

Wu Y, Wang X, Csencsits KL, Haddad A, Walters N, Pascual DW

Abstract

DNA immunization, although attractive, is poor for inducing mucosal immunity, thus limiting its protective value against most infectious agents. To surmount this shortcoming, we devised a method for mucosal transgene vaccination by using an M cell ligand to direct the DNA vaccine to mucosal inductive tissues and the respiratory epithelium. This ligand, reovirus protein final sigma1, when conjugated to polylysine (PL), can bind the apical surface of M cells from nasal-associated lymphoid tissues. Intranasal immunizations with protein final sigma1-PL-DNA complexes produced antigen-specific serum IgG and prolonged mucosal IgA, as well as enhanced cell-mediated immunity, made evident by elevated pulmonary cytotoxic T lymphocyte responses. Therefore, targeted transgene vaccination represents an approach for enabling DNA vaccination of the mucosa.

MeSH Terms
Animals Enzyme-Linked Immunosorbent Assay Gene Transfer Techniques Mice Mice, Inbred BALB C T-Lymphocytes, Cytotoxic/cytology,immunology Vaccines, DNA/administration & dosage,immunology Viral Nonstructural Proteins/administration & dosage,genetics,immunology
Chemicals
Vaccines, DNA Viral Nonstructural Proteins sigma 1NS protein, Reovirus
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wu Y
Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717-3610, USA.
Wang X
Csencsits K L
Haddad A
Walters N
Pascual D W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.161204098
Published
2001-07-31
Epub
2001-00-17
Pages
9318-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55418
Subset
IM
Grants
NIDCR NIH HHS · R01 DE013812 · United States
NIAID NIH HHS · AI 42673 · United States
NIDCR NIH HHS · DE 13812 · United States
NCRR NIH HHS · S10 RR11877 · United States
External Links
PubMed source DOI: 10.1073/pnas.161204098 PMC Full Text View journal details
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