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PMID: 11448992 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p.

The Journal of cell biology ·Vol. 154 ·No. 1 ·2001-07-09 ·Pages 85-94

Huang JN, Park I, Ellingson E, Littlepage LE, Pellman D

Abstract

Cell cycle progression is driven by waves of cyclin expression coupled with regulated protein degradation. An essential step for initiating mitosis is the inactivation of proteolysis mediated by the anaphase-promoting complex/cyclosome (APC/C) bound to its regulator Cdh1p/Hct1p. Yeast APC(Cdh1) was proposed previously to be inactivated at Start by G1 cyclin/cyclin-dependent kinase (CDK). Here, we demonstrate that in a normal cell cycle APC(Cdh1) is inactivated in a graded manner and is not extinguished until S phase. Complete inactivation of APC(Cdh1) requires S phase cyclins. Further, persistent APC(Cdh1) activity throughout G1 helps to ensure the proper timing of Cdc20p expression. This suggests that S phase cyclins have an important role in allowing the accumulation of mitotic cyclins and further suggests a regulatory loop among S phase cyclins, APC(Cdh1), and APC(Cdc20).

MeSH Terms
Anaphase-Promoting Complex-Cyclosome Blotting, Northern Cdc20 Proteins Cdh1 Proteins Cell Cycle Cell Cycle Proteins/metabolism Cloning, Molecular Cyclin B/metabolism Cyclin-Dependent Kinase Inhibitor Proteins Flow Cytometry Fungal Proteins/metabolism,physiology Ligases/metabolism Microscopy, Fluorescence Mitosis Mutagenesis, Site-Directed Mutation Precipitin Tests Protein Structure, Tertiary Saccharomyces cerevisiae Proteins Saccharomycetales Time Factors Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases
Chemicals
CDC20 protein, S cerevisiae CDH1 protein, S cerevisiae CLB2 protein, S cerevisiae Cdc20 Proteins Cdh1 Proteins Cell Cycle Proteins Cyclin B Cyclin-Dependent Kinase Inhibitor Proteins Fungal Proteins SIC1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome Ubiquitin-Protein Ligases Ligases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Huang J N
Department of Pediatric Oncology, The Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
Park I
Ellingson E
Littlepage L E
Pellman D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200102007
Published
2001-07-09
Pages
85-94
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2196868
Subset
IM
Grants
NICHD NIH HHS · K08 HD01203 · United States
NICHD NIH HHS · P30 HD27823-10 · United States
NIGMS NIH HHS · R01 GM55772 · United States
External Links
PubMed source DOI: 10.1083/jcb.200102007 PMC Full Text View journal details
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