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PMID: 11438663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Early expressed Clb proteins allow accumulation of mitotic cyclin by inactivating proteolytic machinery during S phase.

Molecular and cellular biology ·Vol. 21 ·No. 15 ·2001-08-00 ·Pages 5071-81

Yeong FM, Lim HH, Wang Y, Surana U

Abstract

Periodic accumulation and destruction of mitotic cyclins are important for the initiation and termination of M phase. It is known that both APC(Cdc20) and APC(Hct1) collaborate to destroy mitotic cyclins during M phase. Here we show that this relationship between anaphase-promoting complex (APC) and Clb proteins is reversed in S phase such that the early Clb kinases (Clb3, Clb4, and Clb5 kinases) inactivate APC(Hct1) to allow Clb2 accumulation. This alternating antagonism between APC and Clb proteins during S and M phases constitutes an oscillatory system that generates undulations in the levels of mitotic cyclins.

MeSH Terms
Anaphase-Promoting Complex-Cyclosome Blotting, Northern Blotting, Western Cdc20 Proteins Cdh1 Proteins Cell Cycle Proteins/metabolism Cyclin B/metabolism Cyclins/metabolism Flow Cytometry Fungal Proteins/metabolism Gene Deletion Ligases/metabolism Microscopy, Fluorescence Mitosis Models, Biological Mutation Phosphorylation Plasmids/metabolism Protein Binding S Phase Saccharomyces cerevisiae Proteins Time Factors Transcription, Genetic Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases Yeasts/metabolism
Chemicals
CDC20 protein, S cerevisiae CDH1 protein, S cerevisiae CLB2 protein, S cerevisiae Cdc20 Proteins Cdh1 Proteins Cell Cycle Proteins Cyclin B Cyclins Fungal Proteins Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome Ubiquitin-Protein Ligases Ligases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yeong F M
Institute of Molecular and Cell Biology, Singapore 117609, Singapore.
Lim H H
Wang Y
Surana U
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.15.5071-5081.2001
Published
2001-08-00
Pages
5071-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87233
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.21.15.5071-5081.2001 PMC Full Text View journal details
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