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PMID: 11438647 Published · ppublish English Journal Article

Linking the 3' poly(A) tail to the subunit joining step of translation initiation: relations of Pab1p, eukaryotic translation initiation factor 5b (Fun12p), and Ski2p-Slh1p.

Molecular and cellular biology ·Vol. 21 ·No. 15 ·2001-08-00 ·Pages 4900-8

Searfoss A, Dever TE, Wickner R

Abstract

The 3' poly(A) structure improves translation of a eukaryotic mRNA by 50-fold in vivo. This enhancement has been suggested to be due to an interaction of the poly(A) binding protein, Pab1p, with eukaryotic translation initiation factor 4G (eIF4G). However, we find that mutation of eIF4G eliminating its interaction with Pab1p does not diminish the preference for poly(A)(+) mRNA in vivo, indicating another role for poly(A). We show that either the absence of Fun12p (eIF5B), or a defect in eIF5, proteins involved in 60S ribosomal subunit joining, specifically reduces the translation of poly(A)(+) mRNA, suggesting that poly(A) may have a role in promoting the joining step. Deletion of two nonessential putative RNA helicases (genes SKI2 and SLH1) makes poly(A) dispensable for translation. However, in the absence of Fun12p, eliminating Ski2p and Slh1p shows little enhancement of expression of non-poly(A) mRNA. This suggests that Ski2p and Slh1p block translation of non-poly(A) mRNA by an effect on Fun12p, possibly by affecting 60S subunit joining.

MeSH Terms
DEAD-box RNA Helicases Electroporation Eukaryotic Initiation Factor-5 Fungal Proteins/metabolism Gene Deletion Genes, Reporter Kinetics Models, Biological Mutation Peptide Initiation Factors/metabolism Poly A/genetics Poly(A)-Binding Proteins Protein Biosynthesis RNA Helicases/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Ribosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Suppression, Genetic Time Factors
Chemicals
Eukaryotic Initiation Factor-5 Fungal Proteins Peptide Initiation Factors Poly(A)-Binding Proteins RNA, Messenger RNA-Binding Proteins SKI2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Poly A SLH1 protein, S cerevisiae DEAD-box RNA Helicases RNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Searfoss A
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830, USA.
Dever T E
Wickner R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.15.4900-4908.2001
Published
2001-08-00
Pages
4900-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87206
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.21.15.4900-4908.2001 PMC Full Text View journal details
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