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PMID: 11437668 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The NS2 protein generated by the parvovirus minute virus of mice is degraded by the proteasome in a manner independent of ubiquitin chain elongation or activation.

Virology ·Vol. 285 ·No. 2 ·2001-07-05 ·Pages 346-55

Miller CL, Pintel DJ

Abstract

The NS2 protein generated by the parvovirus minute virus of mice is very labile, having a half-life during infection of approximately 90 min. The degradation of NS2 is blocked by proteasome inhibitors but is likely ubiquitin independent: NS2 does not form detectable higher molecular weight ubiquitin-containing conjugates, and NS2 degradation requires neither ubiquitin chain elongation nor intracellular ubiquitin activation. We have also identified a region in the carboxyl half of NS2 that is required for its proteasome-mediated degradation.

MeSH Terms

Amino Acid Chloromethyl Ketones/pharmacology Amino Acid Sequence Animals Binding Sites Cell Line Chromosome Mapping Cysteine Endopeptidases/metabolism Cysteine Proteinase Inhibitors/pharmacology Enzyme Activation Intracellular Fluid/metabolism Mice Minute Virus of Mice/metabolism Molecular Sequence Data Molecular Weight Multienzyme Complexes/antagonists & inhibitors,metabolism Proteasome Endopeptidase Complex Recombinant Fusion Proteins/genetics,metabolism Ubiquitins/metabolism Viral Nonstructural Proteins/genetics,metabolism

Chemicals

Amino Acid Chloromethyl Ketones Cysteine Proteinase Inhibitors Multienzyme Complexes NS1 protein, minute virus of mice Recombinant Fusion Proteins Ubiquitins Viral Nonstructural Proteins benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone Cysteine Endopeptidases Proteasome Endopeptidase Complex

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Miller C L

Department of Molecular Microbiology and Immunology, University of Missouri at Columbia School of Medicine, Columbia, Missouri 65212, USA.

Pintel D J

Article Info

Journal

Virology

Abbr.

Virology

ISSN

0042-6822

DOI

10.1006/viro.2001.0966

Published

2001-07-05

Pages

346-55

Language

English

Region

United States

NLM ID

0110674

Subset

Grants

NIAID NIH HHS · R01 AI21302 · United States

NIAID NIH HHS · T32 AI07276 · United States

External Links

PubMed source DOI: 10.1006/viro.2001.0966 View journal details

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The NS2 protein generated by the parvovirus minute virus of mice is degraded by the proteasome in a manner independent of ubiquitin chain elongation or activation.

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