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PMID: 11425760 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Congruent phylogenies of most common small-subunit rRNA and dissimilatory sulfite reductase gene sequences retrieved from estuarine sediments.

Applied and environmental microbiology ·Vol. 67 ·No. 7 ·2001-07-00 ·Pages 3314-8

Joulian C, Ramsing NB, Ingvorsen K

Abstract

The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and produced Desulfosarcina variabilis-like sequences from the inoculum but Desulfomicrobium baculatum-like sequences from a high dilution in natural media. Related organisms were subsequently cultivated from the site. PCR bias appear to be limited (or very similar) for the two primersets and target genes. However, the DSR primers showed a much higher phylogenetic specificity. DSR gene analysis is thus a promising and specific approach for investigating SRB diversity in complex habitats.

MeSH Terms
DNA, Bacterial/analysis,genetics Deltaproteobacteria/classification,enzymology,genetics Electrophoresis/methods Genes, Bacterial Genes, rRNA Geologic Sediments/microbiology Molecular Sequence Data Oxidoreductases Acting on Sulfur Group Donors/genetics Phylogeny Polymerase Chain Reaction RNA, Ribosomal/genetics Seawater/microbiology Sequence Analysis, DNA Sulfur-Reducing Bacteria/classification,enzymology,genetics
Chemicals
DNA, Bacterial RNA, Ribosomal Oxidoreductases Acting on Sulfur Group Donors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Joulian C
Institute of Biological Sciences, Department of Microbial Ecology, Aarhus University, Ny Munkegade, DK-8000 Aarhus C, Denmark. catherine.joulian@biology.aau.dk
Ramsing N B
Ingvorsen K
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.67.7.3314-3318.2001
Email
catherine.joulian@biology.aau.dk
Published
2001-07-00
Pages
3314-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC93019
Subset
IM
Databases
GENBANK
AF360632, AF360642, AF360643, AF360644, AF360645, AF360646, AF360647, AF360648, AF360649, AF360650, AF360651, AF360652, AF360653, AF360654, AF360655, AF360656, AF360657, AF360658, AF360659, AF360660, AF360661, AF360662, AF360663, AF360664, AF360665, AF360666, AF360667, AF360668, AF360669, AF360670, AF360671, AF360672, AF360673, AF360674, AF360675, AF360676, AF360677, AF360678, AF360679, AF360680, AF360681, AF360682, AF360683, AF360684, AF360685, AF360686, AF360687, AF360688, AF360689, AF360690, AF360691, AF360692, AF360693, AF360694
External Links
PubMed source DOI: 10.1128/AEM.67.7.3314-3318.2001 PMC Full Text View journal details
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