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PMID: 11420431 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers.

Protein science : a publication of the Protein Society ·Vol. 10 ·No. 7 ·2001-07-00 ·Pages 1293-304

Green NS, Reisler E, Houk KN

Abstract

Homobifunctional chemical cross-linking reagents are important tools for functional and structural characterization of proteins. Accurate measures of the lengths of these molecules currently are not available, despite their widespread use. Stochastic dynamics calculations now provide quantitative measures of the lengths, and length dispersions, of 32 widely used molecular rulers. Significant differences from published data have been found. See www.proteinscience.org

MeSH Terms
Amines/chemistry Cross-Linking Reagents/chemistry Models, Molecular Molecular Conformation Molecular Structure Proteins/chemistry Stochastic Processes Sulfhydryl Compounds/chemistry
Chemicals
Amines Cross-Linking Reagents Proteins Sulfhydryl Compounds
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Green N S
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095-1569, USA.
Reisler E
Houk K N
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Article Info
Journal
Protein science : a publication of the Protein Society
Abbr.
Protein Sci
ISSN
0961-8368
DOI
10.1110/ps.51201
Published
2001-07-00
Pages
1293-304
Language
English
Region
United States
NLM ID
9211750
PMCID
PMC2374107
Subset
IM
Grants
NIAMS NIH HHS · R01 AR022031 · United States
NIGMS NIH HHS · R01 GM061402 · United States
NIAMS NIH HHS · AR 22031 · United States
NIGMS NIH HHS · GM 61402 · United States
External Links
PubMed source DOI: 10.1110/ps.51201 PMC Full Text View journal details
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