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PMID: 11418581 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants.

Journal of bacteriology ·Vol. 183 ·No. 14 ·2001-07-00 ·Pages 4386-8

Wagner R, Straub ML, Souciet JL, Potier S, de Montigny J

Abstract

The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.

MeSH Terms
Alleles Carrier Proteins/genetics DNA, Fungal Membrane Transport Proteins/genetics Mutation Mutation, Missense Nucleobase Transport Proteins Plasmids Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins
Chemicals
Carrier Proteins DNA, Fungal FCY2 protein, S cerevisiae Membrane Transport Proteins Nucleobase Transport Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wagner R
Laboratoire de Microbiologie et de Génétique, ESA 7010 Université Louis Pasteur/CNRS, Institut de Botanique, F-67083 Strasbourg Cedex, France.
Straub M L
Souciet J L
Potier S
de Montigny J
References (13)
13 references, click to expand
  1. Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family.
    Mol Membr Biol. 2000 Jan-Mar;17(1):47-57 PMID: 10824738
  2. Characterization of cytosine permeation in Saccharomyces cerevisiae.
    J Bacteriol. 1975 May;122(2):629-41 PMID: 47858
  3. Energy coupling in hypoxanthine transport of yeast. Potentiometric evidence for proton symport and potassium antiport.
    FEBS Lett. 1977 Nov 15;83(2):325-8 PMID: 338354
  4. In vivo and in vitro studies of the purine-cytosine permease of Saccharomyces cerevisiae. Functional analysis of a mutant with an altered apparent transport constant of uptake.
    Eur J Biochem. 1992 Mar 1;204(2):699-704 PMID: 1541283
  5. Determination of a specific region of the purine-cytosine permease involved in the recognition of its substrates.
    Mol Microbiol. 1992 Oct;6(20):2989-97 PMID: 1479890
  6. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1993 Jul 11;21(14):3329-30 PMID: 8341614
  7. Evidence for a dynamic role for proline376 in the purine-cytosine permease of Saccharomyces cerevisiae.
    Eur J Biochem. 1999 Jul;263(1):57-64 PMID: 10429187
  8. Functional analysis of mutated purine-cytosine permease from Saccharomyces cerevisiae. A possible role of the hydrophilic segment 371-377 in the active carrier conformation.
    J Biol Chem. 1997 Apr 11;272(15):9697-702 PMID: 9092500
  9. Potassium transport by amino acid permeases in Saccharomyces cerevisiae.
    J Biol Chem. 1997 May 23;272(21):13647-52 PMID: 9153214
  10. Trinucleotide insertions, deletions, and point mutations in glucose transporters confer K+ uptake in Saccharomyces cerevisiae.
    Mol Cell Biol. 1998 Feb;18(2):926-35 PMID: 9447989
  11. Chimeric purine transporters of Aspergillus nidulans define a domain critical for function and specificity conserved in bacterial, plant and metazoan homologues.
    EMBO J. 1998 Jul 15;17(14):3827-37 PMID: 9670000
  12. Screening of an intragenic second-site suppressor of purine-cytosine permease from Saccharomyces cerevisiae. Possible role of Ser272 in the base translocation process.
    Eur J Biochem. 1999 Feb;260(1):22-30 PMID: 10091580
  13. In vivo phosphorylation of the purine/cytosine permease from the plasma membrane of the yeast Saccharomyces cerevisiae.
    Eur J Biochem. 1996 Jul 15;239(2):439-44 PMID: 8706752
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.14.4386-4388.2001
Published
2001-07-00
Pages
4386-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95330
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.183.14.4386-4388.2001 PMC Full Text View journal details
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