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PMID: 11402015 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Dr operon-associated invasiveness of Escherichia coli from pregnant patients with pyelonephritis.

Infection and immunity ·Vol. 69 ·No. 7 ·2001-07-00 ·Pages 4678-80

Goluszko P, Niesel D, Nowicki B, Selvarangan R, Nowicki S, Hart A, Pawelczyk E, Das M, Urvil P, Hasan R

Abstract

We used a gentamicin protection assay to assess the ability of gestational pyelonephritis isolates of Escherichia coli to invade HeLa cells. The ability to enter HeLa cells was strongly associated with the presence of Dr operons coding for Dr adhesins. In contrast, the nonivasive isolates predominantly expressed papG, coding for P fimbriae.

MeSH Terms
Adhesins, Bacterial/genetics,metabolism,physiology CD55 Antigens/metabolism Escherichia coli/genetics,metabolism,pathogenicity Female Fimbriae, Bacterial/genetics,metabolism HeLa Cells Humans Operon Pregnancy Pregnancy Complications, Infectious/microbiology Pyelonephritis/microbiology
Chemicals
Adhesins, Bacterial CD55 Antigens
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Goluszko P
Departments of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas 77555, USA. pgoluszk@utmb.edu
Niesel D
Nowicki B
Selvarangan R
Nowicki S
Hart A
Pawelczyk E
Das M
Urvil P
Hasan R
References (15)
15 references, click to expand
  1. Virulence factors and O groups of Escherichia coli isolates from patients with acute pyelonephritis, cystitis and asymptomatic bacteriuria.
    Eur J Epidemiol. 1996 Apr;12(2):191-8 PMID: 8817199
  2. Gestational pyelonephritis--associated Escherichia coli isolates represent a nonrandom, closely related population.
    Am J Obstet Gynecol. 1996 Mar;174(3):983-9 PMID: 8633680
  3. Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.
    J Clin Invest. 1997 Apr 1;99(7):1662-72 PMID: 9120010
  4. Acute renal failure in pregnancy.
    Ren Fail. 1997 Mar;19(2):217-20 PMID: 9101594
  5. Dr fimbriae operon of uropathogenic Escherichia coli mediate microtubule-dependent invasion to the HeLa epithelial cell line.
    J Infect Dis. 1997 Jul;176(1):158-67 PMID: 9207362
  6. Molecular cloning and characterization of Dr-II, a nonfimbrial adhesin-I-like adhesin isolated from gestational pyelonephritis-associated Escherichia coli that binds to decay-accelerating factor.
    Infect Immun. 1997 Oct;65(10):4309-18 PMID: 9317041
  7. Lethal outcome of uterine infection in pregnant but not in nonpregnant rats and increased death rate with inhibition of nitric oxide.
    Am J Reprod Immunol. 1997 Oct;38(4):309-12 PMID: 9352021
  8. Role of decay-accelerating factor domains and anchorage in internalization of Dr-fimbriated Escherichia coli.
    Infect Immun. 2000 Mar;68(3):1391-9 PMID: 10678952
  9. Gestational age-dependent distribution of Escherichia coli fimbriae in pregnant patients with pyelonephritis.
    Ann N Y Acad Sci. 1994 Aug 15;730:290-1 PMID: 7915894
  10. Urinary tract infections complicating pregnancy.
    Baillieres Clin Obstet Gynaecol. 1994 Jun;8(2):353-73 PMID: 7924012
  11. Bacterial virulence characteristics of Escherichia coli isolates from first-time urinary tract infection.
    J Infect Dis. 1995 Jun;171(6):1514-21 PMID: 7769286
  12. Association between alpha-hemolysin production and HeLa cell-detaching activity in fecal isolates of Escherichia coli.
    J Clin Microbiol. 1995 Oct;33(10):2707-9 PMID: 8567910
  13. Decreased expression of endometrial decay accelerating factor (DAF), a complement regulatory protein, in patients with luteal phase defect.
    Am J Reprod Immunol. 1995 Oct;34(4):236-40 PMID: 8579761
  14. A novel multiply primed polymerase chain reaction assay for identification of variant papG genes encoding the Gal(alpha 1-4)Gal-binding PapG adhesins of Escherichia coli.
    J Infect Dis. 1996 Apr;173(4):920-6 PMID: 8603972
  15. Urinary tract infections complicating pregnancy.
    Infect Dis Clin North Am. 1997 Mar;11(1):13-26 PMID: 9067782
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.69.7.4678-4680.2001
Email
pgoluszk@utmb.edu
Published
2001-07-00
Pages
4678-80
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC98548
Subset
IM
Grants
NIDDK NIH HHS · 2RO1DK42029 · United States
External Links
PubMed source DOI: 10.1128/IAI.69.7.4678-4680.2001 PMC Full Text View journal details
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