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PMID: 11395453 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Signaling system in Porphyromonas gingivalis based on a LuxS protein.

Journal of bacteriology ·Vol. 183 ·No. 13 ·2001-07-00 ·Pages 3903-9

Chung WO, Park Y, Lamont RJ, McNab R, Barbieri B, Demuth DR

Abstract

The luxS gene of quorum-sensing Vibrio harveyi is required for type 2 autoinducer production. We identified a Porphyromonas gingivalis open reading frame encoding a predicted peptide of 161 aa that shares 29% identity with the amino acid sequence of the LuxS protein of V. harveyi. Conditioned medium from a late-log-phase P. gingivalis culture induced the luciferase operon of V. harveyi, but that from a luxS insertional mutant did not. In P. gingivalis, the expression of luxS mRNA was environmentally controlled and varied according to the cell density and the osmolarity of the culture medium. In addition, differential display PCR showed that the inactivation of P. gingivalis luxS resulted in up-regulation of a hemin acquisition protein and an arginine-specific protease and reduced expression of a hemin-regulated protein, a TonB homologue, and an excinuclease. The data suggest that the luxS gene in P. gingivalis may function to control the expression of genes involved in the acquisition of hemin.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Carbon-Sulfur Lyases Environment Gene Expression Regulation, Bacterial Homoserine/analogs & derivatives,biosynthesis Lactones Molecular Sequence Data Porphyromonas gingivalis/physiology Sequence Homology, Amino Acid Signal Transduction/genetics
Chemicals
Bacterial Proteins Lactones N-octanoylhomoserine lactone Homoserine Carbon-Sulfur Lyases LuxS protein, Bacteria
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chung W O
Department of Oral Biology, University of Washington, Seattle, Washington 98195, USA.
Park Y
Lamont R J
McNab R
Barbieri B
Demuth D R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.13.3903-3909.2001
Published
2001-07-00
Pages
3903-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95272
Subset
IM
Grants
NIDCR NIH HHS · R01 DE011111 · United States
NIDCR NIH HHS · DE12505 · United States
NIDCR NIH HHS · R37 DE011111 · United States
NIDCR NIH HHS · R01 DE012505 · United States
NIDCR NIH HHS · DE11111 · United States
External Links
PubMed source DOI: 10.1128/JB.183.13.3903-3909.2001 PMC Full Text View journal details
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