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PMID: 11390985 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Endogenous Msx1 antisense transcript: in vivo and in vitro evidences, structure, and potential involvement in skeleton development in mammals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 13 ·2001-06-19 ·Pages 7336-41

Blin-Wakkach C, Lezot F, Ghoul-Mazgar S, Hotton D, Monteiro S, Teillaud C, Pibouin L, Orestes-Cardoso S, Papagerakis P, Macdougall M, Robert B, Berdal A

Abstract

Msx1 is a key factor for the development of tooth and craniofacial skeleton and has been proposed to play a pivotal role in terminal cell differentiation. In this paper, we demonstrated the presence of an endogenous Msx1 antisense RNA (Msx1-AS RNA) in mice, rats, and humans. In situ analysis revealed that this RNA is expressed only in differentiated dental and bone cells with an inverse correlation with Msx1 protein. These in vivo data and overexpression of Msx1 sense and AS RNA in an odontoblastic cell line (MO6-G3) showed that the balance between the levels of the two Msx1 RNAs is related to the expression of Msx1 protein. To analyze the impact of this balance in the Msx-Dlx homeoprotein pathway, we analyzed the effect of Msx1, Msx2, and Dlx5 overexpression on proteins involved in skeletal differentiation. We showed that the Msx1-AS RNA is involved in crosstalk between the Msx-Dlx pathways because its expression was abolished by Dlx5. Msx1 was shown to down-regulate a master gene of skeletal cells differentiation, Cbfa1. All these data strongly suggest that the ratio between Msx1 sense and antisense RNAs is a very important factor in the control of skeletal terminal differentiation. Finally, the initiation site for Msx1-AS RNA transcription was located by primer extension in both mouse and human in an identical region, including a consensus TATA box, suggesting an evolutionary conservation of the AS RNA-mediated regulation of Msx1 gene expression.

MeSH Terms
Amino Acid Sequence Animals Cattle Cell Line Chickens Conserved Sequence Embryonic and Fetal Development Evolution, Molecular Gene Expression Regulation, Developmental Heterozygote Homeodomain Proteins/genetics,physiology Humans MSX1 Transcription Factor Mammals Mice Mice, Knockout Molecular Sequence Data Odontoblasts/cytology,physiology Osteogenesis Protein Biosynthesis RNA, Antisense/genetics Rats Sequence Alignment Sequence Homology, Amino Acid Transcription Factors Transcription, Genetic
Chemicals
Homeodomain Proteins MSX1 Transcription Factor Msx1 protein, rat RNA, Antisense Transcription Factors
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Blin-Wakkach C
Laboratory of Biology-Odontology, Institut National de la Santé et de la Recherche Médicale, E 0110, Institut des Cordeliers, IFR 58, 75006 Paris, France. biol_odonto_fr@yahoo.com
Lezot F
Ghoul-Mazgar S
Hotton D
Monteiro S
Teillaud C
Pibouin L
Orestes-Cardoso S
Papagerakis P
Macdougall M
Robert B
Berdal A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.131497098
Email
biol_odonto_fr@yahoo.com
Published
2001-06-19
Epub
2001-00-05
Pages
7336-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC34669
Subset
IM
Databases
GENBANK
AF267728
External Links
PubMed source DOI: 10.1073/pnas.131497098 PMC Full Text View journal details
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