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PMID: 11356954 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The late stage of human immunodeficiency virus type 1 assembly is an energy-dependent process.

Journal of virology ·Vol. 75 ·No. 12 ·2001-06-00 ·Pages 5473-81

Tritel M, Resh MD

Abstract

Several recent studies have indicated the involvement of host cell factors in human immunodeficiency virus type 1 (HIV-1) assembly. To ascertain whether ATP-dependent factors play a role in this process, we quantified virus-like particle (VLP) production by ATP-depleted cells. Pharmacological ATP depletion abrogated VLP production without affecting cell viability or inducing degradation of HIV-1 Gag protein. This effect occurred even when the ATP-depleting agents were added 1 h into the assembly process, and it was reversed by removal of these agents. ATP depletion did not affect Gag membrane binding or multimerization. Density gradient analysis indicated that HIV-1 assembly intermediates were stalled late in the assembly process. This conclusion was further supported by electron microscopy analysis, which revealed a preponderance of plasma membrane-associated stalk-like structures in the ATP-depleted cells. Since no HIV-1 proteins bind or hydrolyze ATP, these findings indicate that an ATP-requiring cellular factor is an obligatory participant late in the HIV-1 assembly process.

MeSH Terms
Adenosine Triphosphate/metabolism Animals COS Cells/metabolism Cell Membrane/metabolism Chlorocebus aethiops Gene Products, gag/metabolism HIV-1/genetics,physiology Humans Transfection Virion/physiology Virus Assembly
Chemicals
Gene Products, gag Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tritel M
Cell Biology Program, Memorial Sloan-Kettering Cancer Center, and Graduate Program in Cell Biology and Genetics, Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA.
Resh M D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.75.12.5473-5481.2001
Published
2001-06-00
Pages
5473-81
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC114259
Subset
IM
Grants
NCI NIH HHS · R01 CA072309 · United States
NCI NIH HHS · CA72309 · United States
External Links
PubMed source DOI: 10.1128/JVI.75.12.5473-5481.2001 PMC Full Text View journal details
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