Regulatory domain of protein stability of human P51/TAP63, a P53 homologue.

Biochemical and biophysical research communications ·Vol. 283 ·No. 5 ·2001-05-25 ·Pages 1135-41

Osada M, Inaba R, Shinohara H, Hagiwara M, Nakamura M, Ikawa Y

Abstract

The amino terminal of human P51/TAp63, a P53 homologue, possesses a transactivation domain involved in the activation of its target genes by binding to DNA elements responsive to the p53 protein family. Using a series of amino terminal deletions, the transactivation domain was mapped between amino acid residues 50 to 69. This domain also regulates protein stability in a proteasome-dependent manner, and Ser51 and Ser68 were found to be essential for this stability. Our results suggest that P51 activity is greatly affected by protein stability.

MeSH Terms

Amino Acid Sequence Animals COS Cells Chlorocebus aethiops Cysteine Endopeptidases/metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Genes, Reporter Genes, Tumor Suppressor Humans Luciferases/genetics,metabolism Molecular Sequence Data Multienzyme Complexes/metabolism Mutagenesis Phosphoproteins Proteasome Endopeptidase Complex Recombinant Proteins/chemistry,metabolism Sequence Alignment Sequence Deletion Sequence Homology, Amino Acid Serine Trans-Activators Transcription Factors Transcriptional Activation Transfection Tumor Suppressor Protein p53/chemistry Tumor Suppressor Proteins

Chemicals

DNA-Binding Proteins Multienzyme Complexes Phosphoproteins Recombinant Proteins TP63 protein, human Trans-Activators Transcription Factors Tumor Suppressor Protein p53 Tumor Suppressor Proteins Serine Luciferases Cysteine Endopeptidases Proteasome Endopeptidase Complex

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Osada M

Human Gene Sciences Center, Medical Research Institute, Tokyo Medical and Dental University, Yushima 1-5-45, Tokyo, Bunkyo-ku, 113-8510, Japan.

Inaba R

Shinohara H

Hagiwara M

Nakamura M

Ikawa Y

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1006/bbrc.2001.4905

Published

2001-05-25

Pages

1135-41

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1006/bbrc.2001.4905 View journal details

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Regulatory domain of protein stability of human P51/TAP63, a P53 homologue.

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