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PMID: 113466 Published · ppublish English Journal Article

Elution of chicken Ig from fixed target cells.

Journal of immunological methods ·Vol. 29 ·No. 2 ·1979-00-00 ·Pages 139-44

Nicolaisen EM, Simonsen M

Abstract

A method is described for preparing fixed chicken peripheral blood cells (PBC) for absorption of allo-antibodies and their subsequent elution. Glutaraldehyde fixation was satisfactory, and with 8 M urea elution haemagglutinating activity was preserved.

MeSH Terms
Adsorption Animals Antibody Specificity Blood Cells Chickens Formaldehyde Glutaral Hemagglutination Tests Immunoglobulin G Immunologic Techniques/methods Isoantibodies Thiocyanates/pharmacology
Chemicals
Immunoglobulin G Isoantibodies Thiocyanates Formaldehyde Glutaral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nicolaisen E M
Simonsen M
Article Info
Journal
Journal of immunological methods
Abbr.
J Immunol Methods
ISSN
0022-1759
DOI
10.1016/0022-1759(79)90063-2
Published
1979-00-00
Pages
139-44
Language
English
Region
Netherlands
NLM ID
1305440
Subset
IM
External Links
PubMed source DOI: 10.1016/0022-1759(79)90063-2 View journal details
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