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PMID: 11331607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

COBRA encodes a putative GPI-anchored protein, which is polarly localized and necessary for oriented cell expansion in Arabidopsis.

Genes & development ·Vol. 15 ·No. 9 ·2001-05-01 ·Pages 1115-27

Schindelman G, Morikami A, Jung J, Baskin TI, Carpita NC, Derbyshire P, McCann MC, Benfey PN

Abstract

To control organ shape, plant cells expand differentially. The organization of the cellulose microfibrils in the cell wall is a key determinant of differential expansion. Mutations in the COBRA (COB) gene of Arabidopsis, known to affect the orientation of cell expansion in the root, are reported here to reduce the amount of crystalline cellulose in cell walls in the root growth zone. The COB gene, identified by map-based cloning, contains a sequence motif found in proteins that are anchored to the extracellular surface of the plasma membrane through a glycosylphosphatidylinositol (GPI) linkage. In animal cells, this lipid linkage is known to confer polar localization to proteins. The COB protein was detected predominately on the longitudinal sides of root cells in the zone of rapid elongation. Moreover, COB RNA levels are dramatically upregulated in cells entering the zone of rapid elongation. Based on these results, models are proposed for the role of COB as a regulator of oriented cell expansion.

MeSH Terms
Amino Acid Sequence Apoproteins Arabidopsis/cytology,genetics,metabolism Arabidopsis Proteins Base Sequence Cell Membrane/metabolism Cell Polarity/physiology Cellulose/metabolism Chromosome Mapping Cloning, Molecular Cytochrome b Group Cytochromes b Gene Expression Regulation, Plant Glycosylphosphatidylinositols/metabolism Membrane Glycoproteins/genetics,metabolism Molecular Sequence Data Mutation Plant Roots/cytology RNA, Plant/metabolism
Chemicals
Apoproteins Arabidopsis Proteins COB protein, Arabidopsis Cytochrome b Group Glycosylphosphatidylinositols Membrane Glycoproteins RNA, Plant Cellulose Cytochromes b
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Schindelman G
Department of Biology, New York University, New York, New York 10003, USA.
Morikami A
Jung J
Baskin T I
Carpita N C
Derbyshire P
McCann M C
Benfey P N
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.879101
Published
2001-05-01
Pages
1115-27
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312689
Subset
IM
Databases
GENBANK
AA556615, AF319663, AI166150, AI416924, AI730765, AI773156, AI861761, AJ006787, AW559872, AW720520, BE130595, H74542
External Links
PubMed source DOI: 10.1101/gad.879101 PMC Full Text View journal details
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