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PMID: 11325974 Published · ppublish English Journal Article

Group A streptococcal genotypes from pediatric throat isolates in Rome, Italy.

Journal of clinical microbiology ·Vol. 39 ·No. 5 ·2001-05-00 ·Pages 1687-90

Dicuonzo G, Gherardi G, Lorino G, Angeletti S, De Cesaris M, Fiscarelli E, Bessen DE, Beall B

Abstract

In a study assessing genetic diversity, 114 group A streptococcus (GAS) isolates were recovered from pediatric pharyngitis patients in Rome, Italy. These isolates comprised 22 different M protein gene (emm) sequence types, 14 of which were associated with a distinct serum opacity factor/fibronectin binding protein gene (sof) sequence type. Isolates with the same emm gene sequence type generally shared a highly conserved chromosomal macrorestriction profile. In three instances, isolates with dissimilar macrorestriction profiles had identical emm types; in each of these cases multilocus sequence typing revealed that isolates with the same emm type were clones having the same allelic profiles. Ninety-eight percent of the pharyngeal isolates had emm types previously found to be highly associated with mga locus gene patterns commonly found in pharyngeal GAS isolates.

MeSH Terms
Adolescent Adult Antigens, Bacterial Bacterial Outer Membrane Proteins/genetics Bacterial Typing Techniques Carrier Proteins/genetics Child Child, Preschool Electrophoresis, Gel, Pulsed-Field Female Genetic Variation Genotype Humans Infant Infant, Newborn Italy Male Peptide Hydrolases/genetics Pharyngitis/microbiology Pharynx/microbiology Polymorphism, Restriction Fragment Length Streptococcal Infections/microbiology Streptococcus pyogenes/classification,genetics,isolation & purification
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Carrier Proteins opacity factor streptococcal M protein Peptide Hydrolases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Dicuonzo G
Department of Medicine and Microbiology, Università Campus Bio-Medico, Rome, Italy.
Gherardi G
Lorino G
Angeletti S
De Cesaris M
Fiscarelli E
Bessen D E
Beall B
References (16)
16 references, click to expand
  1. emm and sof gene sequence variation in relation to serological typing of opacity-factor-positive group A streptococci.
    Microbiology. 2000 May;146 ( Pt 5):1195-209 PMID: 10832648
  2. Association of a serum opacity reaction with serological type in Streptococcus pyogenes.
    J Gen Microbiol. 1961 Jul;25:347-52 PMID: 13707001
  3. Multilocus sequence typing of Streptococcus pyogenes and the relationships between emm type and clone.
    Infect Immun. 2001 Apr;69(4):2416-27 PMID: 11254602
  4. The use of the serum opacity reaction in the typing of group-A streptococci.
    J Med Microbiol. 1973 Feb;6(1):83-90 PMID: 4734813
  5. Application of the polymerase chain reaction to study the M protein(-like) gene family in beta-hemolytic streptococci.
    Med Microbiol Immunol. 1991;180(4):213-27 PMID: 1784271
  6. A review of the correlation of T-agglutination patterns and M-protein typing and opacity factor production in the identification of group A streptococci.
    J Med Microbiol. 1993 May;38(5):311-5 PMID: 8487287
  7. Structural heterogeneity of the emm gene cluster in group A streptococci.
    Mol Microbiol. 1993 May;8(4):707-17 PMID: 8332063
  8. Non-congruent relationships between variation in emm gene sequences and the population genetic structure of group A streptococci.
    Mol Microbiol. 1994 Nov;14(4):619-31 PMID: 7891551
  9. Genetic correlates of throat and skin isolates of group A streptococci.
    J Infect Dis. 1996 Apr;173(4):896-900 PMID: 8603968
  10. Recombinant, octavalent group A streptococcal M protein vaccine.
    Vaccine. 1996 Jul;14(10):944-8 PMID: 8873386
  11. A genetic-based evaluation of the principal tissue reservoir for group A streptococci isolated from normally sterile sites.
    J Infect Dis. 1997 Jul;176(1):177-82 PMID: 9207364
  12. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms.
    Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3140-5 PMID: 9501229
  13. Streptococcal emm types associated with T-agglutination types and the use of conserved emm gene restriction fragment patterns for subtyping group A streptococci.
    J Med Microbiol. 1998 Oct;47(10):893-8 PMID: 9788813
  14. Genetic linkage of exotoxin alleles and emm gene markers for tissue tropism in group A streptococci.
    J Infect Dis. 1999 Mar;179(3):627-36 PMID: 9952369
  15. emm typing and validation of provisional M types for group A streptococci.
    Emerg Infect Dis. 1999 Mar-Apr;5(2):247-53 PMID: 10221877
  16. Contrasting molecular epidemiology of group A streptococci causing tropical and nontropical infections of the skin and throat.
    J Infect Dis. 2000 Oct;182(4):1109-16 PMID: 10979907
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.39.5.1687-1690.2001
Published
2001-05-00
Pages
1687-90
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC88009
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.39.5.1687-1690.2001 PMC Full Text View journal details
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