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题目:: p53-dependent apoptosis induced by proteasome inhibition in mammary epithelial cells.
作者:: MacLaren(A P),Chapman(R S),Wyllie(A H),Watson(C J)
状态:: 发布时间2005-10-13 , 更新时间 2016-11-24
期刊:: Cell Death Differ
摘要:: We have examined the effects of inhibition of the 26S proteasome in a murine mammary cell line, KIM-2 cells using the peptide aldehyde inhibitor MG132. These studies have demonstrated a clear requirement for proteasome function in cell viability. Induction of apoptosis was observed following MG132 treatment in KIM-2 cells and this death was shown to be dependent on the cell actively traversing the cell cycle. KIM-2 cells were generated using a temperature sensitive T-antigen (Tag) and studies at the permissive temperature (33 degrees C) have shown that a Tag binding protein was essential for this apoptotic response. Studies in two additional cell lines, HC11, which is a mammary epithelial cell line carrying mutant p53 alleles and p53 null ES cells suggest that p53 is actively required for the apoptosis induced as a consequence of proteasome inhibition. These results suggest a pivotal role for the 26S proteasome degradation pathway in progression through the cell cycle in proliferating cells.
语言:: eng
DOI:: 10.1038/sj.cdd.4400801

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