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PMID: 11287961 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Functional proteins from a random-sequence library.

Nature ·Vol. 410 ·No. 6829 ·2001-04-05 ·Pages 715-8

Keefe AD, Szostak JW

Abstract

Functional primordial proteins presumably originated from random sequences, but it is not known how frequently functional, or even folded, proteins occur in collections of random sequences. Here we have used in vitro selection of messenger RNA displayed proteins, in which each protein is covalently linked through its carboxy terminus to the 3' end of its encoding mRNA, to sample a large number of distinct random sequences. Starting from a library of 6 x 1012 proteins each containing 80 contiguous random amino acids, we selected functional proteins by enriching for those that bind to ATP. This selection yielded four new ATP-binding proteins that appear to be unrelated to each other or to anything found in the current databases of biological proteins. The frequency of occurrence of functional proteins in random-sequence libraries appears to be similar to that observed for equivalent RNA libraries.

MeSH Terms
ATP-Binding Cassette Transporters Adenosine Triphosphate/metabolism Amino Acid Sequence Binding Sites Carrier Proteins/genetics,metabolism Escherichia coli Escherichia coli Proteins Gene Library Maltose-Binding Proteins Molecular Sequence Data Monosaccharide Transport Proteins Peptide Library Protein Binding Protein Folding Proteins/genetics,isolation & purification,metabolism RNA/genetics Recombinant Fusion Proteins/genetics,metabolism
Chemicals
ATP-Binding Cassette Transporters Carrier Proteins Escherichia coli Proteins Maltose-Binding Proteins Monosaccharide Transport Proteins Peptide Library Proteins Recombinant Fusion Proteins maltose transport system, E coli RNA Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Keefe A D
Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.
Szostak J W
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
0028-0836
Published
2001-04-05
Pages
715-8
Language
English
Region
England
NLM ID
0410462
PMCID
PMC4476321
Subset
IM
Grants
Howard Hughes Medical Institute · United States
Databases
GENBANK
AF306524, AF306525, AF306526, AF306527, AF306528, AF306529
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