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PMID: 11282615 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates.

Applied and environmental microbiology ·Vol. 67 ·No. 4 ·2001-04-00 ·Pages 1628-35

Eaton TJ, Gasson MJ

Abstract

Enterococci are used as starter and probiotic cultures in foods, and they occur as natural food contaminants. The genus Enterococcus is of increased significance as a cause of nosocomial infections, and this trend is exacerbated by the development of antibiotic resistance. In this study, we investigated the incidence of known virulence determinants in starter, food, and medical strains of Enterococcus faecalis, E. faecium, and E. durans. PCR and gene probe strategies were used to screen enterococcal isolates from both food and medical sources. Different and distinct patterns of incidence of virulence determinants were found for the E. faecalis and E. faecium strains. Medical E. faecalis strains had more virulence determinants than did food strains, which, in turn, had more than did starter strains. All of the E. faecalis strains tested possessed multiple determinants (between 6 and 11). E. faecium strains were generally free of virulence determinants, with notable exceptions. Significantly, esp and gelE determinants were identified in E. faecium medical strains. These virulence determinants have not previously been identified in E. faecium strains and may result from regional differences or the evolution of pathogenic E. faecium. Phenotypic testing revealed the existence of apparently silent gelE and cyl genes. In E. faecalis, the trend in these silent genes mirrors that of the expressed determinants. The potential for starter strains to acquire virulence determinants by natural conjugation mechanisms was investigated. Transconjugation in which starter strains acquired additional virulence determinants from medical strains was demonstrated. In addition, multiple pheromone-encoding genes were identified in both food and starter strains, indicating their potential to acquire other sex pheromone plasmids. These results suggest that the use of Enterococcus spp. in foods requires careful safety evaluation.

MeSH Terms
Animals Bacterial Proteins/genetics Conjugation, Genetic DNA, Bacterial/analysis Enterococcus/genetics,pathogenicity Food Microbiology Gram-Positive Bacterial Infections/microbiology Humans Polymerase Chain Reaction Virulence/genetics
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eaton T J
Division of Food Safety Sciences, Institute of Food Research, Norwich Research Park, Conley, Norwich NR4 7UA, United Kingdom. Tracy.Eaton@bbsrc.ac.uk
Gasson M J
References (37)
37 references, click to expand
  1. Main microbial flora present as natural starters in Cebreiro raw cow's-milk cheese (northwest Spain).
    Int J Food Microbiol. 1996 Dec;33(2-3):307-13 PMID: 8930715
  2. Transfer of pheromone-inducible plasmids between Enterococcus faecalis in the Syrian hamster gastrointestinal tract.
    J Infect Dis. 1992 Nov;166(5):1188-91 PMID: 1402034
  3. Gene transfer, gentamicin resistance and enterococci.
    J Hosp Infect. 1997 Aug;36(4):249-59 PMID: 9261754
  4. Characterization of the Enterococcus faecalis alpha C protein homolog. Evidence for the expression of alternate forms in commensal and infection derived isolates.
    Adv Exp Med Biol. 1997;418:1045-8 PMID: 9331830
  5. Aggregation and binding substances enhance pathogenicity in rabbit models of Enterococcus faecalis endocarditis.
    Infect Immun. 1998 Jan;66(1):218-23 PMID: 9423861
  6. In vivo testing of an Enterococcus faecalis efaA mutant and use of efaA homologs for species identification.
    FEMS Immunol Med Microbiol. 1998 Aug;21(4):323-31 PMID: 9753005
  7. Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein.
    Infect Immun. 1999 Jan;67(1):193-200 PMID: 9864215
  8. Enterococci at the crossroads of food safety?
    Int J Food Microbiol. 1999 Mar 1;47(1-2):1-24 PMID: 10357269
  9. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
  10. Enterococcal sex pheromone precursors are part of signal sequences for surface lipoproteins.
    Mol Microbiol. 2000 Jan;35(1):246-7 PMID: 10632894
  11. Bacterial sex pheromone-induced plasmid transfer.
    Cell. 1993 Apr 9;73(1):9-12 PMID: 8462105
  12. Lactic acid bacteria and human clinical infection.
    J Appl Bacteriol. 1993 Aug;75(2):95-107 PMID: 8407678
  13. Plasmid-associated hemolysin and aggregation substance production contribute to virulence in experimental enterococcal endocarditis.
    Antimicrob Agents Chemother. 1993 Nov;37(11):2474-7 PMID: 8285637
  14. The sex pheromone system of Enterococcus faecalis. More than just a plasmid-collection mechanism?
    Eur J Biochem. 1994 Jun 1;222(2):235-46 PMID: 8020463
  15. The pathogenicity of enterococci.
    J Antimicrob Chemother. 1994 Jun;33(6):1083-9 PMID: 7928803
  16. Genetic structure of the Enterococcus faecalis plasmid pAD1-encoded cytolytic toxin system and its relationship to lantibiotic determinants.
    J Bacteriol. 1994 Dec;176(23):7335-44 PMID: 7961506
  17. A plasmid-encoded surface protein on Enterococcus faecalis augments its internalization by cultured intestinal epithelial cells.
    J Infect Dis. 1994 Dec;170(6):1549-56 PMID: 7995995
  18. Cloning of an Enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci.
    Infect Immun. 1995 Feb;63(2):703-6 PMID: 7822045
  19. Virulence of enterococci.
    Clin Microbiol Rev. 1994 Oct;7(4):462-78 PMID: 7834601
  20. Incidence of hemolysin, gelatinase, and aggregation substance among enterococci isolated from patients with endocarditis and other infections and from feces of hospitalized and community-based persons.
    J Infect Dis. 1995 May;171(5):1223-9 PMID: 7751697
  21. Mobilization of vancomycin resistance by transposon-mediated fusion of a VanA plasmid with an Enterococcus faecium sex pheromone-response plasmid.
    Gene. 1996 May 24;171(1):9-17 PMID: 8675038
  22. Plasmid-related transmissibility and multiple drug resistance in Streptococcus faecalis subsp. zymogenes strain DS16.
    Antimicrob Agents Chemother. 1979 Jun;15(6):828-30 PMID: 112916
  23. Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917.
    J Bacteriol. 1982 Dec;152(3):1220-30 PMID: 6292164
  24. Plasmid content of Streptococcus faecalis strain 39-5 and identification of a pheromone (cPD1)-induced surface antigen.
    J Gen Microbiol. 1983 Apr;129(4):1207-15 PMID: 6411857
  25. Modification of Streptococcus faecalis sex pheromones after acquisition of plasmid DNA.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5369-73 PMID: 6412228
  26. Streptococcus faecalis sex pheromone (cAM373) also produced by Staphylococcus aureus and identification of a conjugative transposon (Tn918).
    J Bacteriol. 1985 Jun;162(3):1212-20 PMID: 2987186
  27. High incidence of hemolysin production by Enterococcus (Streptococcus) faecalis strains associated with human parenteral infections.
    J Clin Microbiol. 1987 Aug;25(8):1524-8 PMID: 3114322
  28. Characterization of the human neutrophil response to sex pheromones from Streptococcus faecalis.
    Am J Pathol. 1989 Apr;134(4):797-805 PMID: 2495725
  29. Characterization of a class of nonformylated Enterococcus faecalis-derived neutrophil chemotactic peptides: the sex pheromones.
    Proc Natl Acad Sci U S A. 1990 Jan;87(1):66-70 PMID: 2104986
  30. Movable genetic elements and antibiotic resistance in enterococci.
    Eur J Clin Microbiol Infect Dis. 1990 Feb;9(2):90-102 PMID: 2156704
  31. Technologically important characteristics of Enterococcus isolates from milk and dairy products.
    Int J Food Microbiol. 1990 May;10(3-4):349-52 PMID: 2397162
  32. Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1.
    Mol Microbiol. 1990 Jun;4(6):895-904 PMID: 2120541
  33. Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens.
    Infect Immun. 1991 Jan;59(1):415-20 PMID: 1846126
  34. Aggregation substance of Enterococcus faecalis mediates adhesion to cultured renal tubular cells.
    Infect Immun. 1992 Jan;60(1):25-30 PMID: 1729187
  35. Contribution of the pAD1-encoded cytolysin to the severity of experimental Enterococcus faecalis endophthalmitis.
    Infect Immun. 1992 Jun;60(6):2445-52 PMID: 1587612
  36. Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus.
    FEMS Microbiol Lett. 1992 Jun 1;72(2):195-8 PMID: 1505742
  37. Common themes in microbial pathogenicity revisited.
    Microbiol Mol Biol Rev. 1997 Jun;61(2):136-69 PMID: 9184008
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.67.4.1628-1635.2001
Email
tracy.eaton@bbsrc.ac.uk
Published
2001-04-00
Pages
1628-35
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC92779
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.67.4.1628-1635.2001 PMC Full Text View journal details
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