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题目:: Cell fusion is not involved in the generation of giant cells in the Hodgkin-Reed Sternberg cell line L1236.
作者:: Re(D),Benenson(E),Beyer(M),Gresch(O),Draube(A),Diehl(V),Wolf(J)
状态:: 发布时间2001-03-30 , 更新时间 2006-11-15
期刊:: Am J Hematol
摘要:: The mechanism of multinucleated cell formation in Hodgkin's disease has not yet been elucidated. We asked whether the giant multinucleated cells of the H-RS cell line L1236 develop via fusion of the predominant smaller cells. As a positive control for the fusion assay, human B cells from the B-cell lymphoma cell line BJA-B were split into two fractions, stained with the fluorochromes CMTMR and CMFDA, respectively, and fused using the polyethylene glycol 1500 cell hybridization protocol. Double-stained cells indicating fusion of BJA-B cells were detectable for up to 5 days. In parallel, L1236 cells were split into two fractions, stained with the fluorochromes, and mixed. No double-stained L1236 cells were detected. The same result was obtained when using FACS-sorted small mononuclear L1236 cells. It is thus concluded that the large multinucleated cells of the monoclonal H-RS cell line L1236 have emerged by endomitosis rather than by spontaneous cell fusion.
语言:: eng
DOI:: 10.1002/ajh.1068

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