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PMID: 11274428 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

B lymphocyte-restricted expression of prion protein does not enable prion replication in prion protein knockout mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 7 ·2001-03-27 ·Pages 4034-7

Montrasio F, Cozzio A, Flechsig E, Rossi D, Klein MA, Rülicke T, Raeber AJ, Vosshenrich CA, Proft J, Aguzzi A, Weissmann C

Abstract

Prion replication in spleen and neuroinvasion after i.p. inoculation of mice is impaired in forms of immunodeficiency where mature B lymphocytes are lacking. In spleens of wild-type mice, infectivity is associated with B and T lymphocytes and stroma but not with circulating lymphocytes. We generated transgenic prion protein knockout mice overexpressing prion protein in B lymphocytes and found that they failed to accumulate prions in spleen after i.p. inoculation. We conclude that splenic B lymphocytes are not prion-replication competent and that they acquire prions from other cells, most likely follicular dendritic cells with which they closely associate and whose maturation depends on them.

MeSH Terms
Animals B-Lymphocytes/metabolism Central Nervous System/metabolism Dendritic Cells, Follicular/metabolism Mice Mice, Knockout Mice, Transgenic Prions/genetics,metabolism Spleen/metabolism
Chemicals
Prions
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Montrasio F
Institute of Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
Cozzio A
Flechsig E
Rossi D
Klein M A
Rülicke T
Raeber A J
Vosshenrich C A
Proft J
Aguzzi A
Weissmann C
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.051609398
Published
2001-03-27
Epub
2001-00-13
Pages
4034-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC31174
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.051609398 PMC Full Text View journal details
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