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PMID: 11264310 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for the involvement of Tiam1 in axon formation.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 21 ·No. 7 ·2001-04-01 ·Pages 2361-72

Kunda P, Paglini G, Quiroga S, Kosik K, Caceres A

Abstract

In cultured neurons, axon formation is preceded by the appearance in one of the multiple neurites of a large growth cone containing a labile actin network and abundant dynamic microtubules. The invasion-inducing T-lymphoma and metastasis 1 (Tiam1) protein that functions as a guanosine nucleotide exchange factor for Rac1 localizes to this neurite and its growth cone, where it associates with microtubules. Neurons overexpressing Tiam1 extend several axon-like neurites, whereas suppression of Tiam1 prevents axon formation, with most of the cells failing to undergo changes in growth cone size and in cytoskeletal organization typical of prospective axons. Cytochalasin D reverts this effect leading to multiple axon formation and penetration of microtubules within neuritic tips devoid of actin filaments. Taken together, these results suggest that by regulating growth cone actin organization and allowing microtubule invasion within selected growth cones, Tiam1 promotes axon formation and hence participates in neuronal polarization.

MeSH Terms
Actin Cytoskeleton/physiology Animals Axons/physiology Cell Polarity/physiology Cells, Cultured Fluorescent Antibody Technique Growth Cones/physiology Guanine Nucleotide Exchange Factors Microtubules/physiology Neoplasm Proteins Proteins/physiology Pyramidal Cells/physiology Rats T-Lymphoma Invasion and Metastasis-inducing Protein 1
Chemicals
Guanine Nucleotide Exchange Factors Neoplasm Proteins Proteins T-Lymphoma Invasion and Metastasis-inducing Protein 1 Tiam1 protein, rat
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kunda P
Instituto Mercedes y Martin Ferreyra (INIMEC-CONICET), 5000 Cordoba, Argentina.
Paglini G
Quiroga S
Kosik K
Caceres A
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Published
2001-04-01
Pages
2361-72
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6762399
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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