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PMID: 11259680 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nicotinic cholinergic signaling in hippocampal astrocytes involves calcium-induced calcium release from intracellular stores.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 7 ·2001-03-27 ·Pages 4148-53

Sharma G, Vijayaraghavan S

Abstract

In this report we provide evidence that neuronal nicotinic acetylcholine receptors (nAChRs) are present on hippocampal astrocytes and their activation produces rapid currents and calcium transients. Our data indicate that these responses obtained from astrocytes are primarily mediated by an AChR subtype that is functionally blocked by alpha-bungarotoxin (alpha Bgt) and contains the alpha7 subunit (alpha Bgt-AChRs). Furthermore, their action is unusual in that they effectively increase intracellular free calcium concentrations by activating calcium-induced calcium release from intracellular stores, triggered by influx through the receptor channels. These results reveal a mechanism by which alpha Bgt-AChRs on astrocytes can efficiently modulate calcium signaling in the central nervous system in a manner distinct from that observed with these receptors on neurons.

MeSH Terms
Animals Astrocytes/physiology Bungarotoxins/pharmacology Calcium/metabolism Cells, Cultured Electrophysiology Hippocampus/cytology Rats Receptors, Cholinergic/drug effects,physiology Signal Transduction/drug effects,physiology
Chemicals
Bungarotoxins Receptors, Cholinergic Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sharma G
Department of Physiology and Biophysics, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
Vijayaraghavan S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.071540198
Published
2001-03-27
Epub
2001-00-20
Pages
4148-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC31194
Subset
IM
Grants
NIDA NIH HHS · R01 DA010266 · United States
NIDA NIH HHS · R01 DA 10266 · United States
Corrections
CommentIn
PMID: 11274377
External Links
PubMed source DOI: 10.1073/pnas.071540198 PMC Full Text View journal details
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