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PMID: 11244052 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genomic sequence and transcriptional analysis of a 23-kilobase mycobacterial linear plasmid: evidence for horizontal transfer and identification of plasmid maintenance systems.

Journal of bacteriology ·Vol. 183 ·No. 7 ·2001-04-00 ·Pages 2157-64

Le Dantec C, Winter N, Gicquel B, Vincent V, Picardeau M

Abstract

Linear plasmids were unknown in mycobacteria until recently. Here, we report the complete nucleotide sequence of 23-kb linear plasmid pCLP from Mycobacterium celatum, an opportunistic pathogen. The sequence of pCLP revealed at least 19 putative open reading frames (ORFs). Expression of pCLP genes in exponential-phase cultures was determined by reverse transcriptase PCR (RT-PCR). Twelve ORFs were expressed, whereas no transcription of the 7 other ORFs of pCLP was detected. Five of the 12 transcribed ORFs detected by RT-PCR are of unknown function. Sequence analysis revealed similar loci in both M. celatum pCLP and the Mycobacterium tuberculosis chromosome, including transposase-related sequences. This result suggests horizontal transfer between these two organisms. pCLP also contains ORFs that are similar to genes of bacterial circular plasmids involved in partition (par operon) and postsegregational (pem operon) mechanisms. Functional analysis of these ORFs suggests that they probably carry out similar maintenance roles in pCLP.

MeSH Terms

Amino Acid Sequence Base Sequence Chromosome Mapping Gene Transfer, Horizontal Molecular Sequence Data Mycobacterium/genetics Open Reading Frames Operon Plasmids Transcription, Genetic

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Le Dantec C

Laboratoire de Référence des Mycobactéries, Institut Pasteur, 75724 Paris Cedex 15, France.

Winter N

Gicquel B

Vincent V

Picardeau M

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.183.7.2157-2164.2001

Published

2001-04-00

Pages

2157-64

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC95119

Subset

Databases

GENBANK

AF312688

External Links

PubMed source DOI: 10.1128/JB.183.7.2157-2164.2001 PMC Full Text View journal details

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Genomic sequence and transcriptional analysis of a 23-kilobase mycobacterial linear plasmid: evidence for horizontal transfer and identification of plasmid maintenance systems.

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