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PMID: 11243833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A simple and efficient method to transcribe RNAs with reduced 3' heterogeneity.

Methods (San Diego, Calif.) ·Vol. 23 ·No. 3 ·2001-03-00 ·Pages 201-5

Kao C, Rüdisser S, Zheng M

Abstract

Ribose C2' methoxy groups (-OCH3) on the penultimate nucleotide or the last two nucleotides of DNA templates can dramatically improve the quality of transcripts produced by T7 RNA polymerase. This strategy can be adapted to generate transcripts of varying lengths and will allow greater ease in purification of the products.

MeSH Terms

DNA/genetics DNA-Directed RNA Polymerases/metabolism Genetic Techniques RNA/genetics Ribose/chemistry Transcription, Genetic Viral Proteins

Chemicals

Viral Proteins RNA Ribose DNA bacteriophage T7 RNA polymerase DNA-Directed RNA Polymerases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kao C

Department of Biology, Indiana University, Bloomington, Indiana 47405, USA. ckao@bio.indiana.edu

Rüdisser S

Zheng M

Article Info

Journal

Methods (San Diego, Calif.)

Abbr.

Methods

ISSN

1046-2023

DOI

10.1006/meth.2000.1131

ckao@bio.indiana.edu

Published

2001-03-00

Pages

201-5

Language

English

Region

United States

NLM ID

9426302

Subset

Grants

NIGMS NIH HHS · GM10840 · United States

External Links

PubMed source DOI: 10.1006/meth.2000.1131 View journal details

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