Home LiteratureArticle Details

PMID: 11239399 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SNARE complex oligomerization by synaphin/complexin is essential for synaptic vesicle exocytosis.

Cell ·Vol. 104 ·No. 3 ·2001-02-09 ·Pages 421-32

Tokumaru H, Umayahara K, Pellegrini LL, Ishizuka T, Saisu H, Betz H, Augustine GJ, Abe T

Abstract

Synaphin/complexin is a cytosolic protein that preferentially binds to syntaxin within the SNARE complex. We find that synaphin promotes SNAREs to form precomplexes that oligomerize into higher order structures. A peptide from the central, syntaxin binding domain of synaphin competitively inhibits these two proteins from interacting and prevents SNARE complexes from oligomerizing. Injection of this peptide into squid giant presynaptic terminals inhibited neurotransmitter release at a late prefusion step of synaptic vesicle exocytosis. We propose that oligomerization of SNARE complexes into a higher order structure creates a SNARE scaffold for efficient, regulated fusion of synaptic vesicles.

MeSH Terms

Action Potentials Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Binding, Competitive Carrier Proteins/pharmacology Cell Membrane/metabolism Cloning, Molecular DNA, Complementary/metabolism Decapodiformes/metabolism Dose-Response Relationship, Drug Drosophila Electrophysiology Exocytosis Kinetics Membrane Proteins/pharmacology,physiology Microscopy, Electron Models, Biological Molecular Sequence Data Nerve Tissue Proteins/metabolism,physiology Precipitin Tests Protein Binding Protein Structure, Tertiary Qa-SNARE Proteins Rats Recombinant Proteins/metabolism SNARE Proteins Sequence Homology, Amino Acid Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Time Factors Vesicular Transport Proteins

Chemicals

Adaptor Proteins, Vesicular Transport Carrier Proteins DNA, Complementary Membrane Proteins Nerve Tissue Proteins Qa-SNARE Proteins Recombinant Proteins SNARE Proteins Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Vesicular Transport Proteins complexin I complexin II

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Tokumaru H

Department of Neurobiology, Duke University Medical Center, Box 3209, Durham, NC 27710, USA.

Umayahara K

Pellegrini L L

Ishizuka T

Saisu H

Betz H

Augustine G J

Abe T

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(01)00229-x

Published

2001-02-09

Pages

421-32

Language

English

Region

United States

NLM ID

0413066

Subset

Databases

GENBANK

AB003700

External Links

PubMed source DOI: 10.1016/s0092-8674(01)00229-x View journal details

Analysis Services

SNARE complex oligomerization by synaphin/complexin is essential for synaptic vesicle exocytosis.

Contact

WeChat Official Account