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PMID: 11238894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

VAMP3 null mice display normal constitutive, insulin- and exercise-regulated vesicle trafficking.

Molecular and cellular biology ·Vol. 21 ·No. 5 ·2001-03-00 ·Pages 1573-80

Yang C, Mora S, Ryder JW, Coker KJ, Hansen P, Allen LA, Pessin JE

Abstract

To investigate the physiological function of the VAMP3 vesicle SNARE (v-SNARE) isoform in the regulation of GLUT4 vesicle trafficking, we generated homozygotic VAMP3 null mice by targeted gene disruption. The VAMP3 null mice had typical growth rate and weight gain, with normal maintenance of fasting serum glucose and insulin levels. Analysis of glucose disposal and insulin sensitivity demonstrated normal insulin and glucose tolerance, with no evidence for insulin resistance. Insulin stimulation of glucose uptake in isolated primary adipocytes was essentially the same for the wild-type and VAMP3 null mice. Similarly, insulin-, hypoxia-, and exercise-stimulated glucose uptake in isolated skeletal muscle did not differ significantly. In addition, other general membrane trafficking events including phagocytosis, pinocytosis, and transferrin receptor recycling were also found to be unaffected in the VAMP3 null mice. Taken together, these data demonstrate that VAMP3 function is not necessary for either regulated GLUT4 translocation or general constitutive membrane recycling.

MeSH Terms
Adipocytes/metabolism Animals Blood Glucose/metabolism Blotting, Western Body Weight/genetics Cattle Cells, Cultured DNA, Complementary/metabolism Embryo, Mammalian/metabolism Female Fibroblasts/metabolism Gene Deletion Glucose/pharmacokinetics Glucose Transporter Type 4 Homozygote Hypoxia Insulin/blood,metabolism Male Membrane Proteins/chemistry,genetics Mice Models, Genetic Monosaccharide Transport Proteins/metabolism Muscle Proteins Muscle, Skeletal/metabolism Mutagenesis, Site-Directed Phagocytosis Physical Conditioning, Animal Pinocytosis Protein Isoforms Receptors, Transferrin/metabolism SNARE Proteins Sex Factors Time Factors Tissue Distribution Transferrin/metabolism Vesicle-Associated Membrane Protein 3 Vesicular Transport Proteins
Chemicals
Blood Glucose DNA, Complementary Glucose Transporter Type 4 Insulin Membrane Proteins Monosaccharide Transport Proteins Muscle Proteins Protein Isoforms Receptors, Transferrin SNARE Proteins Slc2a4 protein, mouse Transferrin Vesicle-Associated Membrane Protein 3 Vesicular Transport Proteins Glucose
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yang C
Department of Physiology and Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA.
Mora S
Ryder J W
Coker K J
Hansen P
Allen L A
Pessin J E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.5.1573-1580.2001
Published
2001-03-00
Pages
1573-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC86703
Subset
IM
Grants
NIDDK NIH HHS · R37 DK033823 · United States
NIDDK NIH HHS · R01 DK033823 · United States
NIDDK NIH HHS · DK25295 · United States
NIDDK NIH HHS · R01 DK055811 · United States
NIDDK NIH HHS · DK55811 · United States
NIDDK NIH HHS · DK33823 · United States
External Links
PubMed source DOI: 10.1128/MCB.21.5.1573-1580.2001 PMC Full Text View journal details
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