Home LiteratureArticle Details
PMID: 11226323 Published · ppublish English Journal Article

An orchestrated gene expression component of neuronal programmed cell death revealed by cDNA array analysis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 5 ·2001-02-27 ·Pages 2814-9

Chiang LW, Grenier JM, Ettwiller L, Jenkins LP, Ficenec D, Martin J, Jin F, DiStefano PS, Wood A

Abstract

Programmed cell death (PCD) during neuronal development and disease has been shown to require de novo RNA synthesis. However, the time course and regulation of target genes is poorly understood. By using a brain-biased array of over 7,500 cDNAs, we profiled this gene expression component of PCD in cerebellar granule neurons challenged separately by potassium withdrawal, combined potassium and serum withdrawal, and kainic acid administration. We found that hundreds of genes were significantly regulated in discreet waves including known genes whose protein products are involved in PCD. A restricted set of genes was regulated by all models, providing evidence that signals inducing PCD can regulate large assemblages of genes (of which a restricted subset may be shared in multiple pathways).

MeSH Terms
Algorithms Animals Apoptosis/genetics Cells, Cultured DNA, Complementary Gene Expression Profiling Neurons/metabolism Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Rats
Chemicals
DNA, Complementary
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Chiang L W
Millennium Pharmaceuticals, 640 Memorial Drive, Cambridge, MA 02139, USA. chiang@mpi.com
Grenier J M
Ettwiller L
Jenkins L P
Ficenec D
Martin J
Jin F
DiStefano P S
Wood A
References (27)
27 references, click to expand
  1. Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappaB activation in primary hippocampal neurons.
    J Biol Chem. 1999 Mar 26;274(13):8531-8 PMID: 10085086
  2. Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor.
    Cell. 1999 Mar 19;96(6):857-68 PMID: 10102273
  3. Activation of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease). Oligomerization and direct interaction with histone H1.
    J Biol Chem. 1999 May 14;274(20):13836-40 PMID: 10318789
  4. Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls.
    Genes Dev. 1999 May 15;13(10):1211-33 PMID: 10346810
  5. Mediation by a CREB family transcription factor of NGF-dependent survival of sympathetic neurons.
    Science. 1999 Dec 17;286(5448):2358-61 PMID: 10600750
  6. Gene microarray identification of redox and mitochondrial elements that control resistance or sensitivity to apoptosis.
    Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2680-5 PMID: 10716996
  7. Multiple agents rescue PC12 cells from serum-free cell death by translation- and transcription-independent mechanisms.
    J Neurosci. 1991 Aug;11(8):2552-63 PMID: 1714494
  8. Developmental time course and ionic dependence of kainate-mediated toxicity in rat cerebellar granule cell cultures.
    J Pharmacol Exp Ther. 1991 Jan;256(1):402-11 PMID: 1846423
  9. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  10. Modifications in molecular mechanisms associated with control of cell cycle regulated human histone gene expression during differentiation.
    Cell Biophys. 1989 Dec;15(3):201-23 PMID: 2480181
  11. Analysis of repeating DNA sequences by reassociation.
    Methods Enzymol. 1974;29:363-418 PMID: 4850571
  12. Apoptosis in cerebellar granule cells is blocked by high KCl, forskolin, and IGF-1 through distinct mechanisms of action: the involvement of intracellular calcium and RNA synthesis.
    J Neurosci. 1995 Feb;15(2):1172-9 PMID: 7532699
  13. Programmed cell death and the control of cell survival: lessons from the nervous system.
    Science. 1993 Oct 29;262(5134):695-700 PMID: 8235590
  14. Induction of apoptosis in cerebellar granule neurons by low potassium: inhibition of death by insulin-like growth factor I and cAMP.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):10989-93 PMID: 8248201
  15. The role of inducible transcription factors in apoptotic nerve cell death.
    Brain Res Brain Res Rev. 1995 Jul;21(1):1-28 PMID: 8547952
  16. Apoptosis and the cell cycle.
    Curr Opin Cell Biol. 1995 Dec;7(6):825-34 PMID: 8608013
  17. Potassium deprivation-induced apoptosis of cerebellar granule neurons: a sequential requirement for new mRNA and protein synthesis, ICE-like protease activity, and reactive oxygen species.
    J Neurosci. 1996 Aug 1;16(15):4696-706 PMID: 8764657
  18. Kainate induces apoptosis in neurons.
    Neuroscience. 1996 Oct;74(3):675-83 PMID: 8884764
  19. Metabolic and genetic analyses of apoptosis in potassium/serum-deprived rat cerebellar granule cells.
    J Neurosci. 1996 Dec 1;16(23):7487-95 PMID: 8922404
  20. Insulin-like growth factor and potassium depolarization maintain neuronal survival by distinct pathways: possible involvement of PI 3-kinase in IGF-1 signaling.
    J Neurosci. 1997 Mar 1;17(5):1548-60 PMID: 9030615
  21. A model for p53-induced apoptosis.
    Nature. 1997 Sep 18;389(6648):300-5 PMID: 9305847
  22. Bax deletion further orders the cell death pathway in cerebellar granule cells and suggests a caspase-independent pathway to cell death.
    J Cell Biol. 1997 Oct 6;139(1):205-17 PMID: 9314540
  23. Phosphorylation of c-Jun is necessary for apoptosis induced by survival signal withdrawal in cerebellar granule neurons.
    J Neurosci. 1998 Jan 15;18(2):751-62 PMID: 9425017
  24. To die or not to die: an overview of apoptosis and its role in disease.
    JAMA. 1998 Jan 28;279(4):300-7 PMID: 9450715
  25. Neuronal cell death.
    Neuron. 1998 Apr;20(4):633-47 PMID: 9581757
  26. The Brn-3a transcription factor.
    Int J Biochem Cell Biol. 1998 Nov;30(11):1153-7 PMID: 9839440
  27. Withdrawal of survival factors results in activation of the JNK pathway in neuronal cells leading to Fas ligand induction and cell death.
    Mol Cell Biol. 1999 Jan;19(1):751-63 PMID: 9858598
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.051630598
Email
chiang@mpi.com
Published
2001-02-27
Pages
2814-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC30222
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.051630598 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2