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PMID: 11226186 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Trafficking of phosphatidylinositol 3-phosphate from the trans-Golgi network to the lumen of the central vacuole in plant cells.

The Plant cell ·Vol. 13 ·No. 2 ·2001-02-00 ·Pages 287-301

Kim DH, Eu YJ, Yoo CM, Kim YW, Pih KT, Jin JB, Kim SJ, Stenmark H, Hwang I

Abstract

Very limited information is available on the role of phosphatidylinositol 3-phosphate (PI[3]P) in vesicle trafficking in plant cells. To investigate the role of PI(3)P during the vesicle trafficking in plant cells, we exploited the PI(3)P-specific binding property of the endosome binding domain (EBD) (amino acids 1257 to 1411) of human early endosome antigen 1, which is involved in endosome fusion. When expressed transiently in Arabidopsis protoplasts, a green fluorescent protein (GFP):EBD fusion protein exhibited PI(3)P-dependent localization to various compartments--such as the trans-Golgi network, the prevacuolar compartment, the tonoplasts, and the vesicles in the vacuolar lumen--that varied with time. The internalized GFP:EBD eventually disappeared from the lumen. Deletion experiments revealed that the PI(3)P-dependent localization required the Rab5 binding motif in addition to the zinc finger motif. Overexpression of GFP:EBD inhibited vacuolar trafficking of sporamin but not trafficking of H(+)-ATPase to the plasma membrane. On the basis of these results, we propose that the trafficking of GFP:EBD reflects that of PI(3)P and that PI(3)P synthesized at the trans-Golgi network is transported to the vacuole through the prevacuolar compartment for degradation in plant cells.

MeSH Terms
Arabidopsis/genetics,metabolism Base Sequence Binding Sites Biological Transport, Active DNA, Recombinant/genetics Endosomes/metabolism Membrane Fusion Membrane Proteins/chemistry,genetics,metabolism Models, Biological Phosphatidylinositol Phosphates/metabolism Plants, Genetically Modified Protein Structure, Tertiary Recombinant Fusion Proteins/chemistry,genetics,metabolism Vacuoles/metabolism Vesicular Transport Proteins trans-Golgi Network/metabolism
Chemicals
DNA, Recombinant Membrane Proteins Phosphatidylinositol Phosphates Recombinant Fusion Proteins Vesicular Transport Proteins early endosome antigen 1 phosphatidylinositol 3-phosphate
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kim D H
Department of Molecular Biology, Gyeongsang National University, Chinju, 660-701, Korea.
Eu Y J
Yoo C M
Kim Y W
Pih K T
Jin J B
Kim S J
Stenmark H
Hwang I
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.13.2.287
Published
2001-02-00
Pages
287-301
Language
English
Region
England
NLM ID
9208688
PMCID
PMC102243
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.13.2.287 PMC Full Text View journal details
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